Battiston L, Macagno A, Passamonti S, Micali F, Sottocasa G L
Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Italy.
FEBS Lett. 1999 Jun 25;453(3):351-5. doi: 10.1016/s0014-5793(99)00736-x.
The hypothesis that the uneven distribution of bilirubin in the organism, which occurs in hyperbilirubinemia, could reflect an uneven distribution of bilirubin-binding proteins was tested by searching for peptides containing the bilirubin-binding motif identified in bilitranslocase (Battiston et al., 1998). In the rat, positive proteins bands were found to be present only in the liver, gastric mucosa and central nervous system. The electrophoretic mobilities of the positive compounds in the liver and stomach were identical to that of purified bilitranslocase (38 kDa). In the brain, on the contrary, two peptides were found with molecular masses of 79 and 34 kDa, respectively. Their distribution pattern in the central nervous system was different for each of them.
通过寻找含有在胆红素转运酶中鉴定出的胆红素结合基序的肽段（Battiston等人，1998年），对高胆红素血症时机体中胆红素分布不均可能反映胆红素结合蛋白分布不均这一假说进行了验证。在大鼠中，发现阳性蛋白条带仅存在于肝脏、胃黏膜和中枢神经系统中。肝脏和胃中阳性化合物的电泳迁移率与纯化的胆红素转运酶（38 kDa）相同。相反，在大脑中发现了两种肽段，分子量分别为79 kDa和34 kDa。它们在中枢神经系统中的分布模式各不相同。
