与逆转录病毒样颗粒相关的MSRV相关序列的分子克隆与特性分析

Molecular cloning and characterization of MSRV-related sequences associated with retrovirus-like particles.

作者信息

Komurian-Pradel F, Paranhos-Baccala G, Bedin F, Ounanian-Paraz A, Sodoyer M, Ott C, Rajoharison A, Garcia E, Mallet F, Mandrand B, Perron H

机构信息

Ecole Normale Supérieure de Lyon, UMR 103 CNRS-bioMérieux, 46, Allée d'Italie, Lyon Cedex 07, 69364, France.

出版信息

Virology. 1999 Jul 20;260(1):1-9. doi: 10.1006/viro.1999.9792.

DOI:10.1006/viro.1999.9792

PMID:10405350

Abstract

New sequences have been obtained by successive overlapping RT-PCR extensions from the pol region of a retroviral RNA (multiple sclerosis-associated retroviral element, MSRV) amplified in retrovirus-like particles from patients with multiple sclerosis. gag and pol sequences are related to type C oncoviruses, whereas the env sequence is closer to type D. A tryptophan-like (W) tRNA primer-binding site was identified downstream of the RU5 region in the 5'LTR, and the U3R region cloned in the 3'LTR exhibited potent promoter activity. MSRV clones define a novel family of endogenous elements, HERV-W. From our data, HERV-W RNAs are copackaged in extracellular particles which might be produced by replication-competent or transcomplemented HERV-W copies or by an exogenous member of the HERV-W family.

摘要

通过连续重叠RT-PCR扩增，从多发性硬化症患者的逆转录病毒样颗粒中扩增出的逆转录病毒RNA（多发性硬化症相关逆转录病毒元件，MSRV）的pol区域获得了新序列。gag和pol序列与C型肿瘤病毒相关，而env序列更接近D型。在5'LTR的RU5区域下游鉴定出一个色氨酸样（W）tRNA引物结合位点，克隆到3'LTR中的U3R区域表现出强大的启动子活性。MSRV克隆定义了一个新的内源性元件家族，即HERV-W。根据我们的数据，HERV-W RNA被共包装在细胞外颗粒中，这些颗粒可能由具有复制能力或经反式互补的HERV-W拷贝产生，或者由HERV-W家族的外源性成员产生。