Mordon S, Desmettre T, Devoisselle J M
INSERM-EA2689-IFR22 (French National Institute of Health and Medical Research), University Hospital, Lille, France.
Lasers Surg Med. 1999;24(5):338-45. doi: 10.1002/(sici)1096-9101(1999)24:5<338::aid-lsm4>3.0.co;2-i.
An in vivo study was done to establish if laser-induced damage of the retina could be quantified using fluorescein angiography.
This study was carried out on rabbit eyes (n = 6) with an 810 nm diode laser (spot diameter: 500 microm, pulse duration: 1 second, power: 100 mW-400 mW) adapted on a slit lamp. Fluorescence measurements were performed with a fundus camera connected to a fluorescence imaging system. Fluorescence staining of the retina was evaluated by mathematical modeling. Lesions were correlated to laser parameters and to histologic data.
Image analysis shows that the laser lesions stained progressively. Fluorescence appears first at the borders of the lesion exhibiting a fluorescent ring. A progressive increase of the fluorescence into the central zone is observed. The maximum fluorescence intensity into the center of the laser spot is obtained after a delay depending on the laser energy. Below 100 +/- 20 mW, lesions are detectable by fluorescence imaging only. A fluorescence plateau appears for a threshold light dose above 200 +/- 20 mW. Mathematical modeling demonstrates that quantitative assessment of laser-induced damage to the retina is feasible using fluorescence imaging.
The quantification of fluorescence staining in terms of both intensity and time can contribute to a better quantification of laser-induced damage. At last, since laser damage may mimic naturally occurring pathology, this method should also be considered to quantify different types of lesions.
进行一项体内研究，以确定是否可以使用荧光血管造影术对激光诱导的视网膜损伤进行量化。
本研究在兔眼（n = 6）上进行，使用适配于裂隙灯的810 nm二极管激光（光斑直径：500微米，脉冲持续时间：1秒，功率：100 mW - 400 mW）。使用连接到荧光成像系统的眼底相机进行荧光测量。通过数学建模评估视网膜的荧光染色情况。将病变与激光参数和组织学数据相关联。
图像分析表明，激光损伤部位的染色逐渐加深。荧光首先出现在损伤边界，呈现出荧光环。观察到荧光向中心区域逐渐增强。根据激光能量，在延迟一段时间后可获得激光光斑中心的最大荧光强度。低于100 +/- 20 mW时，仅通过荧光成像可检测到病变。当光剂量阈值高于200 +/- 20 mW时会出现荧光平台期。数学建模表明，使用荧光成像对激光诱导的视网膜损伤进行定量评估是可行的。
从强度和时间方面对荧光染色进行量化有助于更好地量化激光诱导的损伤。最后，由于激光损伤可能类似于自然发生的病变，该方法也应被考虑用于量化不同类型的病变。
