• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Fluorimetric detection of enzymatic activity associated with the human tumor suppressor Fhit protein.

作者信息

Asensio A C, Oaknin S, Rotllán P

机构信息

Departamento de Bioquímica y Biología Molecular, Universidad de La Laguna, 38206 La Laguna, Tenerife, Canary Islands, Spain.

出版信息

Biochim Biophys Acta. 1999 Jul 13;1432(2):396-400. doi: 10.1016/s0167-4838(99)00102-8.

DOI:10.1016/s0167-4838(99)00102-8
PMID:10407161
Abstract

The human tumor suppressor Fhit protein exhibits diadenosine triphosphatase activity, hydrolyzing Ap(3)A to AMP and ADP. We report that Fhit protein efficiently cleaves the fluorogenic Ap(3)A analog diethenoadenosine triphosphate giving support to establish a simple fluorimetric assay for quantification of Fhit enzyme. Fluorimetric assays were initially tested to demonstrate that diethyl pyrocarbonate and suramin inhibit Fhit enzyme.

摘要

相似文献

1
Fluorimetric detection of enzymatic activity associated with the human tumor suppressor Fhit protein.
Biochim Biophys Acta. 1999 Jul 13;1432(2):396-400. doi: 10.1016/s0167-4838(99)00102-8.
2
Biochemical and immunochemical characterisation of human diadenosine triphosphatase provides evidence for its identification with the tumour suppressor Fhit protein.人二腺苷三磷酸酶的生化和免疫化学特性为其与肿瘤抑制因子Fhit蛋白的鉴定提供了证据。
Biochimie. 2006 May;88(5):461-71. doi: 10.1016/j.biochi.2005.10.006. Epub 2005 Nov 15.
3
In vitro inhibition of the enzymatic activity of tumor suppressor FHIT gene product by carcinogenic transition metals.
Chem Res Toxicol. 2002 Mar;15(3):319-25. doi: 10.1021/tx010112j.
4
Phosphorylation of the human Fhit tumor suppressor on tyrosine 114 in Escherichia coli and unexpected steady state kinetics of the phosphorylated forms.人Fhit肿瘤抑制因子在大肠杆菌中酪氨酸114位点的磷酸化及磷酸化形式的意外稳态动力学。
Biochemistry. 2005 Apr 26;44(16):6286-92. doi: 10.1021/bi047670s.
5
Engineering human Fhit, a diadenosine triphosphate hydrolase, into an efficient dinucleoside polyphosphate synthase.将人源二磷酸腺苷水解酶Fhit工程改造为高效的二核苷多磷酸合酶。
J Am Chem Soc. 2004 Aug 11;126(31):9548-9. doi: 10.1021/ja0400640.
6
Human diadenosine triphosphate hydrolase: preliminary characterisation and comparison with the Fhit protein, a human tumour suppressor.
Acta Biochim Pol. 2000;47(2):435-41.
7
Ubc9-induced inhibition of diadenosine triphosphate hydrolase activity of the putative tumor suppressor protein Fhit.泛素结合酶9（Ubc9）诱导对假定的肿瘤抑制蛋白Fhit的三磷酸二腺苷水解酶活性的抑制作用。
Arch Biochem Biophys. 2004 Aug 15;428(2):160-4. doi: 10.1016/j.abb.2004.05.020.
8
Expression and simple, one-step purification of fragile histidine triad (Fhit) tumor suppressor mutant forms in Escherichia coli and their interaction with protoporphyrin IX.脆性组氨酸三联体（Fhit）肿瘤抑制突变体形式在大肠杆菌中的表达、一步简单纯化及其与原卟啉IX的相互作用
Biotechnol Lett. 2007 Jun;29(6):877-83. doi: 10.1007/s10529-007-9322-9. Epub 2007 Feb 20.
9
Control of 5',5'-dinucleoside triphosphate catabolism by APH1, a Saccharomyces cerevisiae analog of human FHIT.由酿酒酵母中人类FHIT类似物APH1对5',5'-二核苷三磷酸分解代谢的调控。
J Bacteriol. 1998 May;180(9):2345-9. doi: 10.1128/JB.180.9.2345-2349.1998.
10
Fhit, a putative tumor suppressor in humans, is a dinucleoside 5',5"'-P1,P3-triphosphate hydrolase.Fhit是一种人类中的假定肿瘤抑制因子，是一种二核苷5'，5''-P1，P3-三磷酸水解酶。
Biochemistry. 1996 Sep 10;35(36):11529-35. doi: 10.1021/bi961415t.

引用本文的文献

1
Fhit-nucleotide specificity probed with novel fluorescent and fluorogenic substrates.用新型荧光和荧光底物探测Fhit核苷酸特异性。
J Biol Chem. 2000 Feb 18;275(7):4555-60. doi: 10.1074/jbc.275.7.4555.