Divine K K, Lewis J L, Grant P G, Bench G
Lovelace Respiratory Research Institute, P.O. Box 5890, Albuquerque, New Mexico 87185, USA.
Chem Res Toxicol. 1999 Jul;12(7):575-81. doi: 10.1021/tx9900268.
Neurotoxicity from chronic metal inhalation has been suggested as an underlying contributor to late-developing neurodegenerative diseases that have symptoms similar to Alzheimer's and Parkinson's syndromes. If inhaled metals contribute to pathogenesis of these diseases, identifying, localizing, and quantitating metal deposition(s) within specific target regions of the central nervous system will be critical to our understanding of the mechanisms. Standard analytical techniques used to date require exposure to extremely high concentrations of metals to meet analytical detection limits in small tissue areas. The relevance to lower-dose environmentally relevant exposures and potential protective barriers is therefore questionable. The feasibility of microbeam particle-induced X-ray emission is investigated as a method for rapidly scanning tissues to study the inhalation of metals, nasal permeability, and central nervous system deposition. The optimal beam spot and analysis time used to image the rat olfactory epithelium to facilitate the rapid detection of aluminum localizations were determined. Measurements of aluminum localizations in rat olfactory bulb and brain sections are also presented.
长期吸入金属导致的神经毒性被认为是导致后期发展的神经退行性疾病的潜在因素，这些疾病的症状类似于阿尔茨海默病和帕金森综合征。如果吸入的金属促成了这些疾病的发病机制，那么在中枢神经系统的特定目标区域识别、定位和定量金属沉积对于我们理解其机制至关重要。迄今为止使用的标准分析技术需要暴露于极高浓度的金属才能在小组织区域达到分析检测限。因此，其与低剂量环境相关暴露及潜在保护屏障的相关性值得怀疑。研究了微束粒子诱导X射线发射作为一种快速扫描组织以研究金属吸入、鼻通透性和中枢神经系统沉积的方法的可行性。确定了用于对大鼠嗅觉上皮进行成像以促进快速检测铝定位的最佳束斑和分析时间。还展示了大鼠嗅球和脑切片中铝定位的测量结果。
