Arun P, Ravikumar A, Leelamma S, Kurup P A
Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram, India.
Indian J Biochem Biophys. 1998 Oct;35(5):308-12.
A procedure for estimation of digoxin in biological samples after adding a known quantity of digoxin followed by extraction, separation by TLC and HPLC is described. The identity of digoxin thus extracted from rat brain has been established by reaction with digoxin antibody and by its inhibition of Na(+)-K+ ATPase activity. The method could be a better substitute to the routine radioimmunoassay as interfering substances are removed by TLC and HPLC.
描述了一种在生物样品中添加已知量地高辛后进行提取、通过薄层色谱法（TLC）和高效液相色谱法（HPLC）分离来估算地高辛的方法。从大鼠脑中提取的地高辛通过与地高辛抗体反应及其对钠钾ATP酶活性的抑制作用得以鉴定。由于干扰物质通过TLC和HPLC被去除，该方法可能是常规放射免疫测定法的更好替代方法。
