Schmitt R, Lesot H, Vonesch J L, Ruch J V
INSERM U424, Institut de Biologie Médicale, Faculté de Médecine, Strasbourg, France.
Int J Dev Biol. 1999 May;43(3):255-60.
Day 14 ICR mouse first lower (M1) and upper molars (M1) as well as heterotopic recombinations of M1 epithelium/M1 mesenchyme and M1 epithelium/M1 mesenchyme were cultured for 6, 8 and 10 days on semi-solid medium. Computer-assisted 3D reconstructions were performed to follow the in vitro development of these explants. In vitro culture of cap-stage molars allowed for the emergence of unequivocal morphological features distinctive for M1 versus M1 including the cusp pattern, cusp inclination and tooth specific chronology for odontoblast and ameloblast terminal differentiations. Both M1 epithelium/M1 mesenchyme and M1 epithelium/M1 mesenchyme recombinations developed according to the known developmental fate of the mesenchyme. Our data demonstrate that the cap-stage dental ecto-mesenchyme not only directs tooth class specific morphogenesis, but also individual molar crown features. Furthermore, the mesenchyme apparently also controls the typical mirror symmetry of right and left handed teeth.
第14天，将ICR小鼠的第一下磨牙（M1）和上磨牙（M1）以及M1上皮/M1间充质和M1上皮/M1间充质的异位重组在半固体培养基上培养6、8和10天。进行计算机辅助的三维重建以跟踪这些外植体的体外发育。帽状期磨牙的体外培养使得能够出现M1与M1不同的明确形态特征，包括尖模式、尖倾斜度以及成牙本质细胞和成釉细胞终末分化的牙齿特异性时间顺序。M1上皮/M1间充质和M1上皮/M1间充质重组均根据间充质已知的发育命运进行发育。我们的数据表明，帽状期牙外胚间充质不仅指导牙齿类别的特异性形态发生，还指导单个磨牙冠特征。此外，间充质显然还控制着左右牙齿典型的镜像对称。
