Weinmann A R, Oliveira M S, Jorge S M, Martins A R
Department of Pediatrics, Faculty of Medicine of Ribeirão Prêto, University of São Paulo, Brazil.
J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):231-6. doi: 10.1016/s0378-4347(99)00155-3.
The simultaneous determination of retinol and tocopherol by isocratic HPLC in 100 microl serum from preterm newborns is described. Retinol (tR 2.02+/-0.04 min) and retinyl acetate were detected fluorometrically, and were baseline-resolved in 4 min. Tocopherol (tR 8.4+/-0.16 min) and tocopheryl acetate were detected by UV absorbance. Intra- and inter-assay RSD were: retinol, 5.6 and 8.1, and tocopherol, 3.6 and 6.7, respectively. This method is fast, selective and highly sensitive for retinol. It permits the measurement of serum concentrations of retinol and tocopherol with good accuracy and precision.
本文描述了采用等度高效液相色谱法同时测定早产儿100微升血清中视黄醇和生育酚的方法。视黄醇（保留时间2.02±0.04分钟）和醋酸视黄酯通过荧光法检测，在4分钟内实现基线分离。生育酚（保留时间8.4±0.16分钟）和醋酸生育酚通过紫外吸光度检测。批内和批间相对标准偏差分别为：视黄醇5.6%和8.1%，生育酚3.6%和6.7%。该方法对视黄醇快速、选择性高且灵敏度高。它能够以良好的准确度和精密度测定血清中视黄醇和生育酚的浓度。
