Biesalski H, Greiff H, Brodda K, Hafner G, Bässler K H
Int J Vitam Nutr Res. 1986;56(4):319-27.
The simultaneous determination of alpha-tocopherol and retinol in human serum is reported. The separation is carried out by means of isocratic HPLC on adsorption columns. UV-Detection is possible by using either one wavelength for both compounds (300 nm), or after a lambda change mode with typical wavelengths for alpha-tocopherol (292 nm) and retinol (325 nm). According to short retention times (10 min) and rapid extraction the method is useful for clinical research and allows about 50 analyses per day and operator. Blood from 176 human volunteers was collected and alpha-tocopherol and retinol levels in serum determined with this method. Statistical evaluation of different selected groups shows typical significant differences of alpha-tocopherol and retinol concentrations in smokers and oral contraceptive users.
本文报道了人血清中α-生育酚和视黄醇的同时测定方法。采用吸附柱等度高效液相色谱法进行分离。可通过两种方式进行紫外检测：对两种化合物均使用一个波长（300nm），或者在波长切换模式下，分别使用α-生育酚的典型波长（292nm）和视黄醇的典型波长（325nm）。由于保留时间短（10分钟）且提取速度快，该方法适用于临床研究，每个操作人员每天大约可进行50次分析。收集了176名人类志愿者的血液，并用该方法测定血清中的α-生育酚和视黄醇水平。对不同选定组别的统计评估显示，吸烟者和口服避孕药使用者的α-生育酚和视黄醇浓度存在典型的显著差异。
