Arbughi T, Bertani F, Celeste R, Tirone P
Milano Research Centre, Milan, Italy.
J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):323-32. doi: 10.1016/s0378-4347(99)00180-2.
Iopiperidol is a non ionic iodinated compound currently under evaluation as a potential contrast medium with anticoagulant property for radiological examinations. An HPLC method for assaying iopiperidol in plasma and urine of rats and humans is described. The analysis is based on the reversed-phase chromatographic separation of iopiperidol and the internal standard (iopamidol) from the endogenous components of the biological fluids, and their detection by UV absorption at 244 nm. The selectivity of the method was satisfactory. The mean absolute recovery was greater than 80%. The precision and accuracy of the analytical methods were in the range 0.3 to 3.3 and -8.5 to +11%, respectively. The detection limits of iopiperidol in plasma (0.1 ml) and urine (0.25 ml) were 0.2 and 0.4 microg/ml, respectively.
碘哌醇是一种非离子型碘化化合物，目前正在作为一种具有抗凝特性的潜在造影剂进行评估，用于放射学检查。本文描述了一种用于测定大鼠和人体血浆及尿液中碘哌醇的高效液相色谱法。该分析基于碘哌醇和内标物（碘帕醇）与生物流体中内源性成分的反相色谱分离，并通过在244nm处的紫外吸收进行检测。该方法的选择性令人满意。平均绝对回收率大于80%。分析方法的精密度和准确度分别在0.3%至3.3%和-8.5%至+11%的范围内。碘哌醇在血浆（0.1ml）和尿液（0.25ml）中的检测限分别为0.2和0.4μg/ml。
