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通过菲咯啉切割确定的30S核糖体亚基中靠近790环的位置。

Positions in the 30S ribosomal subunit proximal to the 790 loop as determined by phenanthroline cleavage.

作者信息

Muth G W, Hennelly S P, Hill W E

机构信息

Department of Chemistry, The University of Montana, Missoula 59812, USA.

出版信息

RNA. 1999 Jul;5(7):856-64. doi: 10.1017/s1355838299990143.

DOI:10.1017/s1355838299990143
PMID:10411129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1369810/
Abstract

Positioning rRNA within the ribosome remains a challenging problem. Such positioning is critical to understanding ribosome function, as various rRNA regions interact to form suitable binding sites for ligands, such as tRNA and mRNA. We have used phenanthroline, a chemical nuclease, as a proximity probe, to help elucidate the regions of rRNA that are near neighbors of the stem-loop structure centering at nt 790 in the 16S rRNA of the Escherichia coli 30S ribosomal subunit. Using phenanthroline covalently attached to a DNA oligomer complementary to nt 787-795, we found that nt 582-584, 693-694, 787-790, and 795-797 were cleaved robustly and must lie within about 15 A of the tethered site at the 5' end of the DNA oligomer, which is adjacent to nt 795 of 16S rRNA.

摘要

将核糖体RNA（rRNA）定位在核糖体内部仍然是一个具有挑战性的问题。这种定位对于理解核糖体功能至关重要，因为各种rRNA区域相互作用形成适合配体（如转运RNA（tRNA）和信使RNA（mRNA））结合的位点。我们使用了邻菲罗啉，一种化学核酸酶，作为邻近探针，以帮助阐明在大肠杆菌30S核糖体亚基的16S rRNA中以核苷酸790为中心的茎环结构附近的rRNA区域。使用与核苷酸787 - 795互补的DNA寡聚物共价连接的邻菲罗啉，我们发现核苷酸582 - 584、693 - 694、787 - 790和795 - 797被强烈切割，并且必定位于与DNA寡聚物5'端连接位点相邻的大约15埃范围内，该连接位点与16S rRNA的核苷酸795相邻。

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本文引用的文献

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Cleavage of a 23S rRNA pseudoknot by phenanthroline-Cu(II).菲咯啉-铜（II）对23S rRNA假结的切割
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Nucleotides in 16S rRNA protected by the association of 30S and 50S ribosomal subunits.由30S和50S核糖体亚基结合所保护的16S核糖体RNA中的核苷酸。
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Neighborhood of 16S rRNA nucleotides U788/U789 in the 30S ribosomal subunit determined by site-directed crosslinking.通过定点交联确定的30S核糖体亚基中16S rRNA核苷酸U788/U789的邻近区域。
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Flexibility of the nascent polypeptide chain within the ribosome--contacts from the peptide N-terminus to a specific region of the 30S subunit.核糖体中新生多肽链的灵活性——肽链N端与30S亚基特定区域的接触。
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Regions of 16S ribosomal RNA proximal to transfer RNA bound at the P-site of Escherichia coli ribosomes.与大肠杆菌核糖体P位点结合的转运RNA近端的16S核糖体RNA区域。
Biochemistry. 1998 Feb 3;37(5):1350-6. doi: 10.1021/bi9720540.
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The path of the growing peptide chain through the 23S rRNA in the 50S ribosomal subunit; a comparative cross-linking study with three different peptide families.50S核糖体亚基中生长肽链通过23S rRNA的途径；与三个不同肽家族的比较交联研究。
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A new model for the three-dimensional folding of Escherichia coli 16 S ribosomal RNA. I. Fitting the RNA to a 3D electron microscopic map at 20 A.大肠杆菌16S核糖体RNA三维折叠的新模型。I. 将RNA与20埃分辨率的三维电子显微镜图谱拟合。
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9
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Biochemistry. 1997 Jul 1;36(26):7951-7. doi: 10.1021/bi9624954.
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