在核糖体中，16S核糖体RNA被在A位点密码子处用菲咯啉-铜（II）修饰的信使核糖核酸切割。
Cleavage of 16S rRNA within the ribosome by mRNA modified in the A-site codon with phenanthroline-Cu(II).
作者信息
Bucklin D J, van Waes M A, Bullard J M, Hill W E
机构信息
Division of Biological Sciences, University of Montana, Missoula, Montana 59812, USA.
出版信息
Biochemistry. 1997 Jul 1;36(26):7951-7. doi: 10.1021/bi9624954.
Cleavage of 16S rRNA was obtained through mRNA modified at position +5 with the chemical cleavage agent 1,10-o-phenanthroline. In the presence of Cu2+, and after addition of reducing agent to the modified mRNA-70S complex, cleavage of proximal nucleotides within the 16S rRNA occurred. Primer extension analysis of 16S rRNA fragments revealed that nucleotides 528-532, 1196, and 1396-1397 were cleaved. Nucleotides 1053-1055 were also cleaved but did not show the same level of specificity as the former. These results provide evidence that at some point in the translation process these regions are all within 15 A of position +5, the A-site codon, on the mRNA.
通过用化学切割剂1,10 - 邻菲啰啉修饰位于+5位置的mRNA来实现16S rRNA的切割。在Cu2+存在的情况下，向修饰后的mRNA - 70S复合物中加入还原剂后，16S rRNA内近端核苷酸发生切割。对16S rRNA片段进行引物延伸分析表明，核苷酸528 - 532、1196以及1396 - 1397被切割。核苷酸1053 - 1055也被切割，但未表现出与前者相同程度的特异性。这些结果提供了证据，表明在翻译过程中的某个时刻，这些区域都位于mRNA上+5位置（A位点密码子）的15埃范围内。
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