Stagliano N E, Pérez-Pinzón M A, Moskowitz M A, Huang P L
Department of Medicine, Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown 02129, USA.
J Cereb Blood Flow Metab. 1999 Jul;19(7):757-61. doi: 10.1097/00004647-199907000-00005.
In a process called ischemic preconditioning, a brief, sublethal ischemic insult protects tissue from subsequent, more severe injury. There have been no reports of rapidly induced ischemic preconditioning. The authors sought to develop a model of cerebral ischemic preconditioning in the mouse that can be applied to transgenic and knockout animals. They found that brief middle cerebral artery (MCA) occlusion only minutes before a severe ischemic insult can induce protection from that insult. Here the investigators describe a mouse model of preconditioning using intraluminal MCA occlusion as both the conditioning and the test stimulus. One or three 5-minute episodes of ischemia given 30 minutes before MCA occlusion for 1 or 24 hours (permanent occlusion) confer significant protection as assessed by infarct volume measurements 24 hours later.
在一种称为缺血预处理的过程中，短暂的、亚致死性缺血损伤可保护组织免受随后更严重的损伤。目前尚无快速诱导缺血预处理的报道。作者试图建立一种可应用于转基因和基因敲除动物的小鼠脑缺血预处理模型。他们发现，在严重缺血损伤前仅几分钟进行短暂的大脑中动脉（MCA）闭塞，可诱导对该损伤的保护作用。在此，研究人员描述了一种使用管腔内MCA闭塞作为预处理和测试刺激的预处理小鼠模型。在MCA闭塞1小时或24小时（永久性闭塞）前30分钟给予一次或三次5分钟的缺血发作，通过24小时后的梗死体积测量评估，可提供显著的保护作用。
