齐墩果酸 - 环糊精包合物对龋齿的影响：体外实验及大鼠龋齿模型研究

Effect of oleanolic acid-cyclodextrin inclusion compounds on dental caries by in vitro experiment and rat-caries model.

作者信息

Kozai K, Suzuki J, Okada M, Nagasaka N

机构信息

Department of Paediatric Dentistry, Hiroshima University, School of Dentistry, Japan.

出版信息

Microbios. 1999;97(388):179-88.

PMID:10413873

Abstract

Earlier work in vitro showed that oleanolic acid (OA) was a potential inhibitor of insoluble glucan (ISG) synthesis from mutans streptococci (MS). In this study, two oleanolic acid-cyclodextrin inclusion compounds (OA-CDs), oleanolic acid-G1-beta-cyclodextrin (OA-G1-beta CD) and oleanolic acid-beta-cyclodextrin (OA-beta CD), were assayed for their effects on ISG synthesis from Streptococcus mutans MT8148R, and on the growth of oral bacteria. OA-beta CD inhibited ISG synthesis by 55.3 and 37.4% at 62.5 and 15.6 micrograms/ml of OA, respectively. Both OA-CDs inhibited the growth of MS, S. sanguis, and S. salivarius at 4 to 8 micrograms/ml of OA. The anticariogenic effect of the OA-beta CD was examined in a rat-caries model. Rats in the infected control groups showed the highest caries score. The infected treatment group B (0.5% OA in diet) showed lower scores than the control group. These results suggest that OA-beta CD is a potential anti-caries agent.

摘要

早期的体外研究表明，齐墩果酸（OA）是变形链球菌（MS）不溶性葡聚糖（ISG）合成的潜在抑制剂。在本研究中，检测了两种齐墩果酸-环糊精包合物（OA-CDs），即齐墩果酸-G1-β-环糊精（OA-G1-β-CD）和齐墩果酸-β-环糊精（OA-β-CD）对变形链球菌MT8148R的ISG合成以及口腔细菌生长的影响。OA-β-CD在OA浓度为62.5和15.6微克/毫升时，分别抑制ISG合成55.3%和37.4%。两种OA-CDs在OA浓度为4至8微克/毫升时均抑制MS、血链球菌和唾液链球菌的生长。在大鼠龋齿模型中检测了OA-β-CD的防龋效果。感染对照组的大鼠龋齿评分最高。感染治疗组B（饮食中含0.5% OA）的评分低于对照组。这些结果表明，OA-β-CD是一种潜在的抗龋剂。