Yazdankhah S P, Sølverød L, Simonsen S, Olsen E
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, Oslo.
Vet Microbiol. 1999 Jun 15;67(2):113-25. doi: 10.1016/s0378-1135(99)00035-8.
An ELISA method based on monodisperse magnetic beads was developed for the detection of Staphylococcus aureus thermostable nuclease (TNase) in composite milk, wherein S. aureus TNase is captured by magnetic beads coated with monoclonal antibodies directed against TNase and subsequently detected by an enzyme-labelled MAb against the same antigen. Sensitivity of the test was approximately 1 ng TNase, which corresponds to the amount of TNase produced and secreted by approximately 10(5) S. aureus per ml. The Immuno Magnetic Separation (IMS)-ELISA detected TNase in samples from which no S. aureus could be demonstrated on culture. The total test time is 3 h and can be performed either on preserved or fresh milk. The method may be automated.
开发了一种基于单分散磁珠的酶联免疫吸附测定（ELISA）方法，用于检测复合牛奶中的金黄色葡萄球菌耐热核酸酶（TNase）。其中，金黄色葡萄球菌TNase被包被有抗TNase单克隆抗体的磁珠捕获，随后用针对相同抗原的酶标记单克隆抗体进行检测。该检测方法的灵敏度约为1 ng TNase，这相当于每毫升约10⁵个金黄色葡萄球菌产生和分泌的TNase量。免疫磁珠分离（IMS）-ELISA检测到了培养时未检出金黄色葡萄球菌的样品中的TNase。总检测时间为3小时，可对保存牛奶或新鲜牛奶进行检测。该方法可实现自动化操作。
