Takeda A, Takeda N
Department of Internal Medicine, Aoto Hospital, Jekei University School of Medicine, Tokyo, Japan.
Ann N Y Acad Sci. 1999 Jun 30;874:427-35. doi: 10.1111/j.1749-6632.1999.tb09256.x.
This investigation focused on whether apoptosis can be observed in some heart diseases. Apoptosis was examined immunochemically using monoclonal antibodies such as p53, Bcl-2 and cyclin E, A, and B1 in parallel with flow cytometry. Left ventricular myocardium was obtained at autopsy from 40 patients with acute myocarditis (AM; N = 10, 6 males, 4 females, mean age 56 +/- 13 years), chronic myocarditis (CM; N = 10, 5 males, 5 females, mean age 48 +/- 16 years), dilated cardiomyopathy (DCM; N = 10, 7 males, 3 females, mean age 60 +/- 11 years), and no heart disease (Cont; N = 10, 5 males, 5 females, mean age 63 +/- 14 years). Cell cycle analysis of myocytes by flow cytometry revealed that the relative content of G2M phase in acute myocarditis was far higher than those in other heart diseases (AM, 12.3 +/- 3.7%; CM, 5.2 +/- 4.5%; DCM, 6.3 +/- 4.0%; Cont, 3.4 +/- 1.8%; Mean +/- SD). Expression of p53 was observed mainly in myocytes from chronic myocarditis. Expression of Bcl-2, on the other hand, was detected in myocytes from acute myocarditis. Results suggest that apoptosis may play some role in the repairing process of myocardial inflammation.
本研究聚焦于是否能在某些心脏病中观察到细胞凋亡。使用诸如p53、Bcl-2和细胞周期蛋白E、A及B1等单克隆抗体，通过免疫化学方法并结合流式细胞术对细胞凋亡进行检测。从40例患者尸检获取左心室心肌组织，其中急性心肌炎（AM；n = 10，男性6例，女性4例，平均年龄56±13岁）、慢性心肌炎（CM；n = 10，男性5例，女性5例，平均年龄48±16岁）、扩张型心肌病（DCM；n = 10，男性7例，女性3例，平均年龄60±11岁）以及无心脏病患者（对照组；n = 10，男性5例，女性5例，平均年龄63±14岁）。通过流式细胞术对心肌细胞进行细胞周期分析显示，急性心肌炎中G2M期的相对含量远高于其他心脏病（AM，12.3±3.7%；CM，5.2±4.5%；DCM，6.3±4.0%；对照组，3.4±1.8%；均值±标准差）。p53的表达主要在慢性心肌炎的心肌细胞中观察到。另一方面，Bcl-2的表达在急性心肌炎的心肌细胞中被检测到。结果表明细胞凋亡可能在心肌炎症的修复过程中发挥一定作用。
