Zhou D, Kintsourashvili E, Mamujee S, Vacek I, Sun A M
Department of Physiology, Faculty of Medicine, University of Toronto, Ontario, Canada.
Ann N Y Acad Sci. 1999 Jun 18;875:208-18. doi: 10.1111/j.1749-6632.1999.tb08505.x.
In this study, insulin secretion function of INS-1 cells immunoisolated in microcapsules was evaluated. Following encapsulation, the immunoisolated INS-1 cells continued to propagate and flourish within the microcapsules during the entire two-month in vitro incubation period. The insulin secretion from encapsulated INS-1 cells following seven days of in vitro culture increased from 1.6 +/- 0.2 ng/2h/10(6) cells in a glucose-free medium to 11.5 +/- 2.1 ng/2h/10(6) cells at 16.7 mM glucose. In vivo, transplants of 1.2 x 10(7) cells into each of six diabetic C57BL/6 mice resulted in the restoration of normoglycemia in all graft recipients for up to 60 days post transplantation. Most capsules recovered from two animals 30 days post transplantation were free of cell overgrowth and physically intact. Immunostaining for insulin of the cells within the recovered capsules clearly indicated the presence of insulin. The presented data demonstrate the potential use of an immunoisolated beta-cell line for the treatment of diabetes.
在本研究中，对微胶囊中免疫隔离的INS-1细胞的胰岛素分泌功能进行了评估。封装后，免疫隔离的INS-1细胞在整个两个月的体外培养期内在微胶囊中持续增殖并生长良好。体外培养7天后，封装的INS-1细胞在无葡萄糖培养基中的胰岛素分泌从1.6±0.2 ng/2h/10⁶细胞增加到16.7 mM葡萄糖时的11.5±2.1 ng/2h/10⁶细胞。在体内，将1.2×10⁷个细胞移植到6只糖尿病C57BL/6小鼠中的每只体内，导致所有移植受体在移植后长达60天内血糖恢复正常。移植后30天从两只动物体内回收的大多数胶囊没有细胞过度生长且物理完整。对回收胶囊内细胞进行胰岛素免疫染色清楚地表明存在胰岛素。所呈现的数据证明了免疫隔离的β细胞系在糖尿病治疗中的潜在用途。
