Calahorra B, Campanero M A, Sádaba B, Azanza J R
Department of Clinical Pharmacology, Clinica Universitaria de Navarra, Pamplona, Spain.
Biomed Chromatogr. 1999 Jun;13(4):272-5. doi: 10.1002/(SICI)1099-0801(199906)13:4<272::AID-BMC842>3.0.CO;2-0.
A simple, rapid and reproducible high-performance liquid chromatographic method for the quantitative determination of cefepime in human plasma was developed. Ceftazidime was used as internal standard. Chromatography was performed on a reversed-phase encapped column (Hypersil BDS C18). The samples, after protein precipitation, were eluted with a mobile phase of acetonitrile-acetate buffer, pH 4 (2.8:97.2, v/v). The detection wavelength was 254 nm. The limit of quantitation of cefepime was 0.5 microgram/mL and only 0.5 mL of plasma sample was required for the determination. The average cefepime recoveries over a concentration range of 0.5-500 micrograms/mL ranged from 98 to 104%. Precision and accuracy did not exceed 5%.
建立了一种简单、快速且可重复的高效液相色谱法，用于定量测定人血浆中的头孢吡肟。以头孢他啶为内标。色谱分析在反相封端柱（Hypersil BDS C18）上进行。样品经蛋白沉淀后，用乙腈 - 醋酸盐缓冲液（pH 4，2.8:97.2，v/v）作为流动相洗脱。检测波长为254 nm。头孢吡肟的定量限为0.5微克/毫升，测定仅需0.5毫升血浆样品。在0.5 - 500微克/毫升的浓度范围内，头孢吡肟的平均回收率为98%至104%。精密度和准确度不超过5%。
