Emara S, Saleh G, Fathy M, Bakr M A
Department of Analytical Chemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Biomed Chromatogr. 1999 Jun;13(4):299-303. doi: 10.1002/(SICI)1099-0801(199906)13:4<299::AID-BMC867>3.0.CO;2-Y.
A high-performance liquid chromatographic system with automated precolumn extraction was developed for the determination of propofol in human serum. Propofol of directly injected serum sample was enriched on a protein-coated mu Bondapak phenyl precolumn while serum constituents such as proteins and salts were eluted to waste. Thereafter, using an on-line column-switching system, the drug was quantitatively transferred and separated on a second analytical column followed by spectrophotometric determination at 270 nm. Good precision, accuracy and linearity were obtained over a range of 30-3000 ng/mL propofol in human serum. The developed method proved to be fast, simple, reproducible, reliable and therefore convenient for propofol monitoring from serum. The recovery of propofol in serum samples from the lowest to the highest concentration ranged from 96.84 to 100.16% (n = 5). The assay was applied to study the pharmacokinetic of the drug in six women undergoing elective caesarean section under general anaesthesia induced with a single intravenous bolus dose of propofol (2.5 mg/kg).
开发了一种具有自动柱前萃取功能的高效液相色谱系统，用于测定人血清中的丙泊酚。直接进样的血清样品中的丙泊酚在涂有蛋白质的μ Bondapak苯基预柱上富集，而血清成分如蛋白质和盐则被洗脱至废液中。此后，使用在线柱切换系统，将药物定量转移至第二根分析柱上进行分离，然后在270 nm处进行分光光度测定。在人血清中丙泊酚浓度为30 - 3000 ng/mL范围内，该方法具有良好的精密度、准确度和线性。所开发的方法快速、简单、可重复、可靠，因此便于从血清中监测丙泊酚。血清样品中丙泊酚的回收率从最低浓度到最高浓度范围为96.84%至100.16%（n = 5）。该测定法用于研究在单次静脉推注丙泊酚（2.5 mg/kg）诱导全身麻醉下接受择期剖宫产的6名女性中该药物的药代动力学。
