Cussonneau Xavier, De Smet Els, Lantsoght Kristof, Salvi Jean-Paul, Bolon-Larger Magali, Boulieu Roselyne
Université de Lyon, and Hospices Civils de Lyon, Laboratoire de Pharmacocinétique Clinique, Hôpital Cardiologique, Bron, France.
J Pharm Biomed Anal. 2007 Jul 27;44(3):680-2. doi: 10.1016/j.jpba.2006.10.020. Epub 2006 Nov 28.
A selective and sensitive high-performance liquid chromatographic method for the analysis of propofol in biological samples was developed. Propofol and thymol (internal standard) were analysed on a Purospher RP-18 endcapped (75 mmx4 mm, 3 microm) stationary phase using acetonitrile and water (65:35, v/v) as eluents at a flow rate of 0.6 mL/min. The excitation and emission wavelengths were 276 and 310 nm, respectively. Sample treatment consisted of deproteinization by acetonitrile containing the internal standard and direct injection of the supernatant. Mean analytical recovery were 105% (CV 2.0%) at concentrations ranging from 0.05 to 10 mg/L. The quantification limit was 3 ng/mL for a 500 microL sample plasma volume and 5 ng/mL for a 500 microL blood sample. The intra-day and inter-day precisions were lower than 5.5% for three concentrations assessed (0.05, 1.0 and 10.0 mg/L). Considering the column size and the flow rate, the separation was achieved with an analysis time less than 6 min with a reduced consumption of solvent. This rapid HPLC method using a simple treatment procedure is sensitive enough for monitoring propofol in human biological samples.
建立了一种用于分析生物样品中丙泊酚的选择性和灵敏的高效液相色谱法。丙泊酚和百里酚（内标）在Purospher RP - 18封端（75 mm×4 mm，3微米）固定相上进行分析，使用乙腈和水（65:35，v/v）作为洗脱剂，流速为0.6 mL/min。激发波长和发射波长分别为276和310 nm。样品处理包括用含内标的乙腈进行脱蛋白处理，然后直接进样上清液。在0.05至10 mg/L的浓度范围内，平均分析回收率为105%（CV 2.0%）。对于500 μL样品血浆体积，定量限为3 ng/mL，对于500 μL血样，定量限为5 ng/mL。在所评估的三个浓度（0.05、1.0和10.0 mg/L）下，日内和日间精密度均低于5.5%。考虑到柱尺寸和流速，在不到6分钟的分析时间内实现了分离，同时溶剂消耗减少。这种采用简单处理程序的快速高效液相色谱法对于监测人体生物样品中的丙泊酚足够灵敏。
