Kawano Y, Takaue Y, Watanabe T, Abe T, Okamoto Y, Iwai A, Iwai T, Watanabe A, Ito E, Makimoto A, Nakagawa R, Watanabe H, Sato J, Suenaga K, Suzuya H, Ohnishi T, Kanamaru S, Kaneko M, Kuroda Y
Department of Pediatrics, University of Tokushima, Japan.
Cancer Res. 1999 Jul 15;59(14):3321-4.
The advantages/disadvantages of the use of peripheral blood stem cells (PBSCs) for allogeneic transplantation still need to be clarified, particularly in children. We compared the kinetics, efficacy, and safety of PBSC mobilization by granulocyte colony-stimulating factor (G-CSF) and collection by apheresis between healthy pediatric and adult donors. A total of 19 pediatric (median age, 6 years) and 25 adult healthy donors (median age, 37 years) were given 10 micro/kg/day of G-CSF for 5 consecutive days for PBSC mobilization, which were harvested by apheresis on days 5 and/or 6. All of the donors tolerated the whole procedures. Serum trough levels of G-CSF determined by ELISA were significantly lower in the 16 pediatric donors evaluated than in adults (n = 16) on days 3 and 4 (P < 0.05). Although the WBC counts on days 4 and 5 were significantly higher in adults than in children (P = 0.006 and 0.004, respectively), the numbers of circulating CD34+ cells/unit of blood were identical. The number of blood CD34+ cells collected per unit of blood processed was identical in both donor populations. We propose that PBSCs could be effectively mobilized and collected in small children so that they could be donors for adult patients.
使用外周血干细胞（PBSCs）进行同种异体移植的优缺点仍有待明确，尤其是在儿童中。我们比较了健康儿童和成人供体中粒细胞集落刺激因子（G-CSF）动员PBSC的动力学、疗效和安全性以及通过单采术进行采集的情况。共有19名儿科供体（中位年龄6岁）和25名成人健康供体（中位年龄37岁）连续5天接受10微克/千克/天的G-CSF用于PBSC动员，并在第5天和/或第6天通过单采术采集。所有供体均耐受整个过程。在第3天和第4天，通过ELISA测定的16名接受评估的儿科供体的G-CSF血清谷值水平显著低于成人（n = 16）（P < 0.05）。虽然成人在第4天和第5天的白细胞计数显著高于儿童（分别为P = 0.006和0.004），但每单位血液中循环CD34+细胞的数量相同。两个供体群体中每单位处理血液收集的血液CD34+细胞数量相同。我们提出，幼儿中的PBSCs可以有效地动员和采集，从而使其能够成为成年患者的供体。
