Reichel M, Gillert E, Breitenlohner I, Repp R, Greil J, Beck J D, Fey G H, Marschalek R
University of Erlangen-Nürnberg, Germany.
Cancer Res. 1999 Jul 15;59(14):3357-62.
Chromosomal translocations t(4;11)(q21;q23) are associated with a group of acute lymphoblastic leukemias with very poor prognosis. From the complete sequences of the breakpoint cluster regions of the human MLL and AF-4 translocation partner genes, a novel set of 66 oligonucleotides that facilitates the rapid identification of translocation breakpoints by PCR analysis of genomic DNA was designed. For each breakpoint, a pair of optimally snited primers can be assigned, which improves the monitoring of the disease during treatment. Comparison of the breakpoints with the corresponding parental sequences also contributes to our better understanding of the illegitimate recombination events leading to these translocations.
染色体易位t(4;11)(q21;q23)与一组预后极差的急性淋巴细胞白血病相关。根据人类MLL和AF - 4易位伙伴基因的断点簇区域的完整序列，设计了一组66个新型寡核苷酸，通过对基因组DNA进行PCR分析，有助于快速鉴定易位断点。对于每个断点，可以指定一对最佳匹配的引物，这有助于在治疗期间对疾病进行监测。将断点与相应的亲本序列进行比较，也有助于我们更好地理解导致这些易位的异常重组事件。
