Divekar A A, Gogate A S, Shivkar L K
Department of Microbiology, LTM Medical College, Sion, Mumbai, India.
Sex Transm Dis. 1999 Jul;26(6):358-63. doi: 10.1097/00007435-199907000-00009.
Gonococcal isolates were differentiated based on susceptibility pattern, penicillinase production (PPNG or non-PPNG), serogroup, auxotype, protein, and plasmid profile. The association between serogroup and auxotype and PPNG was determined.
Women attending tertiary level health centers and the sexually transmitted disease (STD) clinic in Mumbai, India, were screened for Neisseria gonorrhoeae. Minimal inhibitory concentration testing was performed according to National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Auxotypes, serogroups, protein profile, and plasmid content were also studied.
Of the 33 isolates, 16 (48.5%) were resistant to penicillin, and 28 (84.8%) showed a chromosomally mediated resistance to tetracycline. Five (15.2%) isolates showed resistance to ciprofloxacin, whereas 12 (36.4%) showed a reduced susceptibility. Twenty-seven (81.8%) isolates belonged to the WI serogroup, and 15 (46.7%) were penicillinase producers (PPNG). Seventeen (51.5%) isolates were of the nonrequiring auxotype, whereas seven (21.2%) were proline requiring. Fifteen (55.6%) of the isolates belonged to the nonrequiring-WI auxotype/serogroup (A/S) class. Ten of the PPNG isolates possessed the 4.4 MDa plasmid, whereas four had the 3.2 MDa plasmid. Increases in the molecular weight of the major outer membrane protein were observed.
A high prevalence of chromosomal resistance to penicillin and tetracycline was observed. The 4.4 MDa plasmid was the most prevalent among the PPNG isolates. We observed ciprofloxacin resistance, which has not been reported in previous studies in India. The nonrequiring auxotype was the most prevalent, followed by the proline requiring auxotype. WI serogroup was the most commonly observed among the isolates studied. The nonrequiring/WI A/S class was the most prevalent among the PPNG.
根据药敏模式、青霉素酶产生情况（产青霉素酶淋球菌或非产青霉素酶淋球菌）、血清群、菌型、蛋白及质粒图谱对淋球菌分离株进行鉴别。确定血清群与菌型及产青霉素酶淋球菌之间的关联。
对印度孟买三级医疗中心和性传播疾病（STD）门诊的女性进行淋病奈瑟菌筛查。根据美国国家临床实验室标准委员会（NCCLS）指南进行最低抑菌浓度测试。还对菌型、血清群、蛋白图谱及质粒含量进行了研究。
在33株分离株中，16株（48.5%）对青霉素耐药，28株（84.8%）显示对四环素具有染色体介导的耐药性。5株（15.2%）分离株对环丙沙星耐药，而12株（36.4%）显示敏感性降低。27株（81.8%）分离株属于WI血清群，15株（46.7%）为青霉素酶生产者（产青霉素酶淋球菌）。17株（51.5%）分离株为非必需菌型，而7株（21.2%）为脯氨酸需求菌型。15株（55.6%）分离株属于非必需-WI菌型/血清群（A/S）类别。10株产青霉素酶淋球菌分离株携带4.4 MDa质粒，而4株携带3.2 MDa质粒。观察到主要外膜蛋白分子量增加。
观察到对青霉素和四环素的染色体耐药性普遍存在。4.4 MDa质粒在产青霉素酶淋球菌分离株中最为常见。我们观察到环丙沙星耐药性，此前在印度的研究中尚未报道过。非必需菌型最为常见，其次是脯氨酸需求菌型。WI血清群是所研究分离株中最常观察到的。非必需/WI A/S类别在产青霉素酶淋球菌中最为常见。
