Sasamura T, Matsuda A, Kokuba Y
Infusion Research Department, Hoechst Marion Roussel Ltd., Saitama, Japan.
Arzneimittelforschung. 1999 Jun;49(6):541-3. doi: 10.1055/s-0031-1300458.
The effects of a nutrition therapy with D-methionine (Met)-containing solution were investigated in cell cultures of the AH109A cell line. The growth of AH109A hepatoma cells in culture media with D-Met-supplemented medium, L-Met-supplemented medium (control) and Met-free medium was compared. The D-Met-supplemented medium inhibited the cell growth to an extent similar to that manifested in the Met-free medium. The total free amino acid concentrations in the control medium decreased by approximately 40% on day 6 post-culture. However, the free amino acid concentrations in D-Met-supplemented and Met-free media did not change. Furthermore, alanine, which was not added to RPMI-1640, was detected in the control medium on day 6 post-culture. These results suggest the possibility of application of D-Met-containing solution to cancer patients receiving total parenteral nutrition.
在AH109A细胞系的细胞培养中研究了含D-蛋氨酸（Met）溶液的营养疗法的效果。比较了AH109A肝癌细胞在补充D-Met的培养基、补充L-Met的培养基（对照）和无Met培养基中的生长情况。补充D-Met的培养基对细胞生长的抑制程度与无Met培养基中表现出的程度相似。培养6天后，对照培养基中的总游离氨基酸浓度下降了约40%。然而，补充D-Met的培养基和无Met培养基中的游离氨基酸浓度没有变化。此外，培养6天后在对照培养基中检测到未添加到RPMI-1640中的丙氨酸。这些结果表明含D-Met溶液应用于接受全胃肠外营养的癌症患者的可能性。
