Gantzer C, Menard D, Lina B, Maul A, Le Guyader S, Thouvenot D, Aymard M, Schwartzbrod L, Kopecka H
Laboratoire de Virologie, Faculté de Pharmacie, Nancy.
Zentralbl Hyg Umweltmed. 1999 Jun;202(1):29-39.
A multi-centric study was carried out in three laboratories, to evaluate the efficiency of a standardized kit for the detection of enterovirus genome in wastewater. Twenty one samples of 20 liters of wastewater were analyzed before and after concentration through glass wool. Each sample was analyzed with the Amplicor kit as well as with techniques developed independently in each laboratory. The results show that the Amplicor kit is well suited to the detection of enterovirus genome in treated wastewater. The results may be compared to those obtained with semi-nested RT-PCR techniques used in each laboratory. However, the Amplicor kit technique is more simple and has the advantage of providing a standardized technique useful for comparative studies. During this work it was observed that the sensitivity of the detection of infectious viruses and virus genome was improved when concentrated samples were used for analysis.
在三个实验室开展了一项多中心研究，以评估一种用于检测废水中肠道病毒基因组的标准化试剂盒的效率。对21个20升的废水样本在通过玻璃棉浓缩前后进行了分析。每个样本均使用Amplicor试剂盒以及各实验室独立开发的技术进行分析。结果表明，Amplicor试剂盒非常适合检测经处理废水中的肠道病毒基因组。这些结果可与各实验室使用半巢式RT-PCR技术获得的结果进行比较。然而，Amplicor试剂盒技术更为简单，且具有提供一种适用于比较研究的标准化技术的优势。在这项工作中观察到，使用浓缩样本进行分析时，传染性病毒和病毒基因组检测的灵敏度有所提高。
