Schulze-Röbbecke R, Hartemann P, Fimmers R, Hagenau C
Institut für Hygiene, University of Düsseldorf, Germany.
Zentralbl Hyg Umweltmed. 1999 Jun;202(1):51-9.
We compared four methods for the cultural recovery of legionellae from naturally contaminated water samples: (A) inoculating 1 and 0.1 ml directly on the selective solid growth medium, (B) filtration through cellulose nitrate membrane filters and inoculating the resuspended residue corresponding to 100 and 10 ml of the original sample on the medium, (C) filtration through polycarbonate filters and inoculating the resuspended residue corresponding to 100 and 10 ml of the original sample, and (D) filtration of 100 and 10 ml through cellulose nitrate filters and incubating the filter directly on the medium. Of the water samples tested in parallel, 103 samples tested positive for legionellae in at least two of these methods. The mean number of CFU/ml recovered by methods A, B, C, and D werte 5.45, 1.85, 1.70, and 2.26, respectively. The differences between the methods proved to be highly significant except for methods B and C (p = 0.112), the results of which correlated closely with each other (r = +0.96). In comparison with method A the recovery rates of methods B, C, and D were found to be 60, 57, and 69%, respectively. We recommend the use of method A for the recovery of legionellae at concentrations of > or = 1 CFU/ml. If legionellae below this concentration are to be detected we recommend method D as the easiest and most sensitive technique.
(A) 将1毫升和0.1毫升直接接种在选择性固体生长培养基上；(B) 通过硝酸纤维素膜过滤器过滤，并将相当于100毫升和10毫升原始样品的重悬残渣接种在培养基上；(C) 通过聚碳酸酯过滤器过滤，并将相当于100毫升和10毫升原始样品的重悬残渣接种在培养基上；(D) 将100毫升和10毫升通过硝酸纤维素过滤器过滤，并将过滤器直接在培养基上孵育。在平行测试的水样中，有103个样品在这些方法中的至少两种方法中检测出军团菌呈阳性。方法A、B、C和D回收的每毫升菌落形成单位（CFU）平均数分别为5.45、1.85、1.70和2.26。除方法B和C外（p = 0.112），各方法之间的差异被证明具有高度显著性，方法B和C的结果彼此密切相关（r = +0.96）。与方法A相比，方法B、C和D的回收率分别为60%、57%和69%。对于浓度≥1 CFU/ml的军团菌回收，我们推荐使用方法A。如果要检测低于此浓度的军团菌，我们推荐方法D，因为它是最简单且最灵敏的技术。
