Szewzyk R, Allestam G, Stenström T A
National Bacteriological Laboratory, Stockholm, Sweden.
Zentralbl Hyg Umweltmed. 1991 Nov;192(3):258-63.
A higher recovery of Legionella bacteria from water samples was achieved using black filters that were directly placed on agar plates after filtration, compared to the traditional method using white filters that are shaken in buffer after filtration. Up to 100 times more Legionella colonies/100 ml were found using black filters compared to the traditional method. The two filtration methods revealed, however, no big differences with pure culture experiments. Treatment with acid solution, directly on the filters without dilution of the concentrated sample, effectively reduced the number of accompanying bacteria.
与传统方法（使用白色滤膜，过滤后在缓冲液中振荡）相比，使用黑色滤膜过滤水样后直接置于琼脂平板上，可实现更高的军团菌回收率。与传统方法相比，使用黑色滤膜发现的军团菌菌落数/100毫升高达100倍。然而，两种过滤方法在纯培养实验中未显示出太大差异。直接在滤膜上用酸溶液处理，不稀释浓缩样品，可有效减少伴随细菌的数量。
