Kang T H, Pae H O, Jeong S J, Yoo J C, Choi B M, Jun C D, Chung H T, Miyamoto T, Higuchi R, Kim Y C
College of Pharmacy, Wonkwang University, Iksan, Korea.
Planta Med. 1999 Jun;65(5):400-3. doi: 10.1055/s-1999-14014.
Bioassay-guided fractionation of an H2O extract of Artemisia feddei has furnished an inducible nitric oxide synthase (iNOS) inhibitory coumarin, scopoletin (1) and one of the inactive sesquiterpenes, achillin (2). Compound 1 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was due to suppression of iNOS mRNA and iNOS protein, as determined by Northern and Western blotting, respectively.
对费德蒿水提取物进行生物活性导向的分离，得到了一种可诱导型一氧化氮合酶（iNOS）抑制性香豆素——东莨菪素（1）和一种无活性倍半萜——洋艾素（2）。化合物1在经γ干扰素（IFN-γ）加脂多糖（LPS）刺激的小鼠巨噬细胞样RAW 264.7细胞中，呈剂量依赖性地抑制一氧化氮（NO）合成。通过Northern印迹和Western印迹分别测定，1对NO合成的抑制作用是由于iNOS mRNA和iNOS蛋白的表达受到抑制。
