Peeters C C, Claassen I J, Schuller M, Kersten G F, van der Voort E M, Poolman J T
Laboratory for Vaccine development and Immune Mechanisms, National Institute of Public Health and Environment, Bilthoven, The Netherlands.
Vaccine. 1999 Jun 4;17(20-21):2702-12. doi: 10.1016/s0264-410x(99)00011-0.
In this study we compare different vaccine formulations containing meningococcal PorA outer membrane protein; purified PorA, outer membrane vesicles (OMV) and immune-stimulating complexes (iscom). Bactericidal antibodies could be generated by the OMV and iscom formulation but not with purified PorA using either A1PO4 or Quil-A as adjuvant. OMV and iscom formulations revealed similar immunogenicity when tested in a dose response manner, with respect to bactericidal as well as OMV-binding antibodies. The anti-OMV IgG subclass response induced by PorA in OMV formulation was found in all subclasses IgG1, IgG2a, IgG2b, IgG3. OMP-iscoms induced very high IgG1 anti-OMV antibodies but almost no IgG3 response. Also, OMP-iscoms appeared to be a potent inducer of antibodies directed against linear peptides corresponding to surface exposed loops of PorA. In addition, iscoms as well as purified PorA with Quil-A as adjuvant (but not with A1PO4) induced high levels of antibodies against purified PorA. In summary, in addition to the OMV formulation, only iscoms containing PorA are able to generate an anamnestic and bactericidal antibody response.
在本研究中，我们比较了含有脑膜炎球菌PorA外膜蛋白的不同疫苗制剂；纯化的PorA、外膜囊泡（OMV）和免疫刺激复合物（iscom）。使用A1PO4或Quil - A作为佐剂时，OMV和iscom制剂可产生杀菌抗体，但纯化的PorA则不能。当以剂量反应方式进行测试时，就杀菌抗体以及OMV结合抗体而言，OMV和iscom制剂显示出相似的免疫原性。在OMV制剂中，PorA诱导的抗OMV IgG亚类反应在所有亚类IgG1、IgG2a、IgG2b、IgG3中均有发现。OMP - iscoms诱导产生非常高的IgG1抗OMV抗体，但几乎没有IgG3反应。此外，OMP - iscoms似乎是针对与PorA表面暴露环相对应的线性肽的抗体的有效诱导剂。另外，iscoms以及以Quil - A作为佐剂（而非A1PO4）的纯化PorA诱导产生了高水平的抗纯化PorA抗体。总之，除了OMV制剂外，只有含有PorA的iscoms能够产生回忆性和杀菌性抗体反应。
