Naess L M, Oftung F, Aase A, Wetzler L M, Sandin R, Michaelsen T E
Department of Vaccinology, National Institute of Public Health, Oslo, Norway.
Infect Immun. 1998 Mar;66(3):959-65. doi: 10.1128/IAI.66.3.959-965.1998.
We have analyzed human T-cell responses in parallel with serum immunoglobulin G (IgG) antibody levels after systemic vaccination with the Norwegian group B Neisseria meningitidis outer membrane vesicle (OMV) vaccine. Ten adult volunteers, with no or very low levels of serum IgG antibodies against meningococci, received three doses intramuscularly of the OMV vaccine (at weeks 0, 6, and 46). T-cell proliferation against the OMV vaccine, purified outer membrane proteins (PorA and PorB), and control antigens (Mycobacterium bovis BCG vaccine and tetanus toxoid) was measured by thymidine incorporation of peripheral blood mononuclear cells before and after vaccination. The results showed that vaccination with OMV elicits strong primary and booster T-cell responses specific to OMV as well as the PorA (class 1) protein and significant, but markedly lower, responses against the PorB (class 3) protein. The median responses to OMV and PorA were 26 and 16 times the prevaccination levels, respectively. Most of the vaccinees showed low T-cell responses against OMV and PorA before vaccination, and the maximum T-cell responses to all vaccine antigens were usually obtained after the second vaccine dose. We found a positive correlation between T-cell responses and anti-OMV IgG antibody levels (r = 0.50, P < 0.0001, for OMV and PorA). In addition, we observed a progressive increase in the percentage of CD45R0+ (memory) CD4-positive T cells (P = 0.002). In conclusion, we have shown that the Norwegian OMV vaccine against meningococcal B disease induced antigen-specific T-cell responses, kinetically accompanied by serum IgG responses, and that vaccination increased the proportion of memory T-helper cells.
我们分析了挪威B群脑膜炎奈瑟菌外膜囊泡（OMV）疫苗全身接种后人体T细胞反应与血清免疫球蛋白G（IgG）抗体水平的情况。10名成年志愿者，其血清中抗脑膜炎球菌IgG抗体水平为无或极低，接受了3剂OMV疫苗的肌肉注射（分别在第0、6和46周）。通过接种前后外周血单核细胞的胸腺嘧啶核苷掺入法，测定针对OMV疫苗、纯化外膜蛋白（PorA和PorB）以及对照抗原（牛分枝杆菌卡介苗和破伤风类毒素）的T细胞增殖情况。结果显示，接种OMV疫苗可引发针对OMV以及PorA（1类）蛋白的强烈初次和加强T细胞反应，而针对PorB（3类）蛋白的反应虽显著但明显较低。对OMV和PorA的反应中位数分别是接种前水平的26倍和16倍。大多数接种者在接种前对OMV和PorA的T细胞反应较低，对所有疫苗抗原的最大T细胞反应通常在第二剂疫苗接种后获得。我们发现T细胞反应与抗OMV IgG抗体水平之间存在正相关（针对OMV和PorA，r = 0.50，P < 0.0001）。此外，我们观察到CD45R0 +（记忆）CD4阳性T细胞的百分比逐渐增加（P = 0.002）。总之，我们表明挪威的抗B群脑膜炎球菌病OMV疫苗可诱导抗原特异性T细胞反应，在动力学上伴有血清IgG反应，并且接种疫苗增加了记忆性辅助性T细胞的比例。
