Chung J G
Department of Medicine, China Medical College, Taichung, Taiwan, Republic of China.
Food Chem Toxicol. 1999 Apr;37(4):327-34. doi: 10.1016/s0278-6915(99)00011-3.
Paeonol was used to determine any effects on the N-acetyltransferase (NAT) activity in human colon tumour cells as measured by HPLC exhibited for the amounts of N-acetyl-2-aminofluorene (AAF) and N-acetyl-p-aminobenzoic acid (N-Ac-PABA) and remaining 2-aminofluorene (AF) and p-aminobenzoic acid (PABA). The NAT activity in the human colon tumour intact cells and cytosols was promoted by paeonol in a dose-dependent manner, that is, the higher the concentrations of paeonol, the higher the promotion of NAT activity. The apparent Vmax values from NAT of human colon tumour cells were also promoted by paeonol in cytosols and in intact cells. The data also demonstrated that co-treatment with paeonol in the media an increase in AF-DNA adduct formation was seen in the human colon tumour cells. This report is the first demonstration to show paeonol did promote human colon tumour cell NAT activity and AF-DNA adduct formation.
丹皮酚用于测定其对人结肠肿瘤细胞中N - 乙酰转移酶（NAT）活性的影响，该活性通过高效液相色谱法（HPLC）测定N - 乙酰 - 2 - 氨基芴（AAF）和N - 乙酰 - 对氨基苯甲酸（N - Ac - PABA）的量以及剩余的2 - 氨基芴（AF）和对氨基苯甲酸（PABA）来衡量。丹皮酚以剂量依赖性方式促进人结肠肿瘤完整细胞和胞质溶胶中的NAT活性，即丹皮酚浓度越高，对NAT活性的促进作用越强。丹皮酚还促进了人结肠肿瘤细胞胞质溶胶和完整细胞中NAT的表观Vmax值。数据还表明，在培养基中与丹皮酚共同处理时，人结肠肿瘤细胞中AF - DNA加合物的形成增加。本报告首次证明丹皮酚确实促进了人结肠肿瘤细胞的NAT活性和AF - DNA加合物的形成。
