Lu Hsueh-Fu, Wu Hsi-Chin, Hsia Te-Chun, Chen Wen-Chi, Hung Chi-Fu, Chung Jing-Gung
Department of Urology, China Medical College Hospital, 2 Yuh-Der Rd, Taichung 400, Taiwan, Republic of China.
J Appl Toxicol. 2002 Jan-Feb;22(1):37-44. doi: 10.1002/jat.824.
In this study, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) were used to determine the inhibition of arylamine N-acetyltransferase (NAT) activity and DNA adduct formation in human bladder tumour cell line T-24. The activity of NAT was measured by high-performance liquid chromatography, assaying for the amounts of N-acetyl-2-aminofluorene and N-acetyl-p-aminobenzoic acid and remaining 2-aminofluorene and p-aminobenzoic acid. Human bladder tumour cell line T-24 cytosols and intact cells were used for examining NAT activity and carcinogen-DNA adduct formation. The results demonstrated that NAT activity and 2-aminofluorene-DNA adduct formation in human bladder tumour cells were inhibited and decreased by BHA and BHT in a dose-dependent manner. The effects of BHA and BHT on the values of the apparent K(m) and V(max) also were determined in both systems examined. The results indicated that BHA and BHT decreased the apparent values of K(m) and V(max) from human bladder tumour cells in both cytosol and intact cells.
在本研究中，使用丁基羟基茴香醚（BHA）和丁基羟基甲苯（BHT）来测定它们对人膀胱肿瘤细胞系T-24中芳胺N-乙酰转移酶（NAT）活性及DNA加合物形成的抑制作用。通过高效液相色谱法测定NAT的活性，分析N-乙酰-2-氨基芴和N-乙酰对氨基苯甲酸的量以及剩余的2-氨基芴和对氨基苯甲酸的量。用人膀胱肿瘤细胞系T-24的胞质溶胶和完整细胞来检测NAT活性和致癌物-DNA加合物的形成。结果表明，BHA和BHT以剂量依赖的方式抑制并降低了人膀胱肿瘤细胞中的NAT活性和2-氨基芴-DNA加合物的形成。还在两个检测系统中测定了BHA和BHT对表观K(m)和V(max)值的影响。结果表明，BHA和BHT降低了胞质溶胶和完整细胞中人膀胱肿瘤细胞的K(m)和V(max)的表观值。
