Li A P, Gorycki P D, Hengstler J G, Kedderis G L, Koebe H G, Rahmani R, de Sousas G, Silva J M, Skett P
In Vitro Technologies, Baltimore, MD 21227, USA.
Chem Biol Interact. 1999 Jun 1;121(1):117-23. doi: 10.1016/s0009-2797(99)00081-2.
Successful cryopreservation of freshly isolated hepatocytes would significantly decrease the need for freshly-procured livers for the preparation of hepatocytes for experimentation. Hepatocytes can be prepared, cryopreserved, and used for experimentation as needed at different times after isolation. Cryopreservation is especially important for research with human hepatocytes because of the limited availability of fresh human livers. Based on the cumulative experience of this international expert panel, a consensus was reached on the various aspects of hepatocyte cryopreservation, including cryopreservation and thawingprocedures and applications of the cryopreserved hepatocytes. Key to successful cryopreservation includes slow addition of cryopreservants, controlled-rate freezing with adjustment for the heat of crystallization, storage at -150 degrees C, and rapid thawing. There is a general consensus that cryopreserved hepatocytes are useful for short-term xenobiotic metabolism and cytotoxicity evaluation.
成功冷冻保存新鲜分离的肝细胞将显著减少为实验制备肝细胞而对新鲜获取肝脏的需求。肝细胞可以在分离后的不同时间根据需要进行制备、冷冻保存并用于实验。由于新鲜人类肝脏的可用性有限，冷冻保存对于人类肝细胞研究尤为重要。基于该国际专家小组的累积经验，在肝细胞冷冻保存的各个方面达成了共识，包括冷冻保存和解冻程序以及冷冻保存肝细胞的应用。成功冷冻保存的关键包括缓慢添加冷冻保护剂、控制速率冷冻并调整结晶热、在-150摄氏度下储存以及快速解冻。普遍的共识是，冷冻保存的肝细胞可用于短期异生物质代谢和细胞毒性评估。
