伯克霍尔德菌分泌外产物所需遗传位点的分子特征分析
Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomallei.
作者信息
DeShazer D, Brett P J, Burtnick M N, Woods D E
机构信息
Genetics and Physiology Branch, Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702, USA.
出版信息
J Bacteriol. 1999 Aug;181(15):4661-4. doi: 10.1128/JB.181.15.4661-4664.1999.
Abstract
Previous studies have demonstrated that Burkholderia pseudomallei secretes protease, lipase, and phospholipase C (PLC) into the extracellular milieu, but their mechanisms of secretion and roles in pathogenesis have not been elucidated. In this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and PLC.
摘要
先前的研究表明，伯克霍尔德菌可将蛋白酶、脂肪酶和磷脂酶C（PLC）分泌到细胞外环境中，但其分泌机制及其在发病机制中的作用尚未阐明。在本研究中，我们分离并鉴定了29个无法分泌蛋白酶、脂肪酶和PLC的转座子突变体。
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