Mongkolsuk S, Rabibhadana S, Vattanaviboon P, Loprasert S
Laboratory of Biotechnology, Chulabhorn Research Institute, Lak si, Bangkok, Thailand.
Gene. 1994 May 27;143(1):145-6. doi: 10.1016/0378-1119(94)90620-3.
We have modified the positive-selection cloning vector pUN121 to expand the numbers of unique cloning sites by insertion of a multiple cloning site into the lambda cI gene without disrupting its repressor function, resulting in plasmid pSKM10. Plasmid pSKM10 has seven restriction enzyme sites suitable for general cloning purposes. A mobilizable version (pSKM11) of pSKM10 was constructed by insertion of the IncP mob sequence which permitted mobilization of the plasmid into a wide variety of Gram- bacteria.
我们对正向选择克隆载体pUN121进行了改造，通过在λ cI基因中插入多克隆位点来增加独特克隆位点的数量，同时不破坏其阻遏功能，从而得到质粒pSKM10。质粒pSKM10有七个适合一般克隆用途的限制性酶切位点。通过插入IncP mob序列构建了pSKM10的可移动版本（pSKM11），该序列允许将质粒转移到多种革兰氏阴性菌中。
