Török G, Péter A, Gaucher A, Wakselman M, Mazaleyrat J P, Armstrong D W
Department of Inorganic and Analytical Chemistry, Attila József University, Szeged, Hungary.
J Chromatogr A. 1999 Jun 18;846(1-2):83-91. doi: 10.1016/s0021-9673(98)01081-4.
The high-performance liquid chromatographic enantioresolution of free and N- and/or C-protected derivatives of (R,S)-2',1':1,2;1",2":3,4-dinaphthcyclohepta-1,3-diene-6-aminometh yl-6- carboxylic acid (beta 2-Bin) by direct and indirect methods is reported. The direct separation was carried out on native and different derivatized beta-cyclodextrin-bonded phases. The indirect resolution was achieved by applying pre-column derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide. The effects of different parameters such as the mobile phase composition and the structures of the compounds on the enantiomeric resolution are discussed.
报道了通过直接和间接方法对（R，S）-2'，1':1,2；1"，2":3,4-二萘并环庚-1,3-二烯-6-氨基甲基-6-羧酸（β2-Bin）的游离及N-和/或C-保护衍生物进行高效液相色谱对映体拆分。直接分离在天然及不同衍生化的β-环糊精键合相上进行。间接拆分通过使用1-氟-2,4-二硝基苯基-5-L-丙氨酸酰胺进行柱前衍生化来实现。讨论了不同参数如流动相组成和化合物结构对对映体拆分的影响。
