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Indirect high-performance liquid chromatographic separation of stereoisomers of beta-alkyl-substituted amino acids by the application of (S)-N-(4-nitrophenoxycarbonyl)phenylalanine methoxyethyl ester as chiral derivatizing agent.

作者信息

Vékes Erika, Török Gabriella, Péter Antal, Sapi János, Tourwé Dirk

机构信息

Department of Inorganic and Analytical Chemistry, University of Szeged, Hungary.

出版信息

J Chromatogr A. 2002 Mar 8;949(1-2):125-39. doi: 10.1016/s0021-9673(01)01455-8.

DOI:10.1016/s0021-9673(01)01455-8
PMID:11999729
Abstract

The indirect high-performance liquid chromatographic enantioresolution of beta-alkyl-substituted analogues of tyrosine, phenylalanine, 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid and tryptophan is reported. (S)-N-(4-Nitrophenoxycarbonyl)phenylalanine methoxyethyl ester, a recently developed chiral derivatizing agent, was used for pre-column derivatization of the investigated analytes. The diastereoisomers formed were analysed under reversed-phase conditions. The effects of parameters such as the amount and type of the organic modifier and the type of the stationary phase on the resolution and retention of the derivatives were investigated. Chromatographic conditions were found for the separation of all four stereoisomers of each analyte.

摘要

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