采用高效液相色谱法测定人体体液中的曲伐沙星。
Determination of trovafloxacin in human body fluids by high-performance liquid chromatography.
作者信息
Borner K, Hartwig H, Lode H
机构信息
Institute for Clinical Chemistry and Pathobiochemistry, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Germany.
出版信息
J Chromatogr A. 1999 Jun 18;846(1-2):175-80. doi: 10.1016/s0021-9673(99)00247-2.
For the quantitative determination of trovafloxacin (a new naphthyridinone antibacterial agent) in serum and urine a simple isocratic HPLC method with fluorimetric detection is described. Serum was deproteinised with a mixture of acetonitrile and perchloric acid. The protein-free extract was separated on a reversed-phase column (Nucleosil 100-5 C18) and quantified by means of fluorescence (excitation 275 nm, emission 405 nm). The mobile phase consisted of a mixture of 250 ml acetonitrile and 750 ml distilled water containing 10 mmol/l tetrabutylammonium phosphate. Urine was diluted with 0.25 mol/l phosphoric acid 1:20 (v/v) which was adjusted to pH 3.6 with sodium hydroxide solution. Diluted urine samples were separated on a cation-exchange column (Nucleosil 100-5 SA) and also detected by means of fluorescence. Trovafloxacin was sufficiently separated from endogenous compounds. Results of validation are given. The method was applied successfully to a study of healthy volunteers.
本文描述了一种采用荧光检测的简单等度高效液相色谱法，用于定量测定血清和尿液中的曲伐沙星（一种新型萘啶酮类抗菌剂）。血清用乙腈和高氯酸的混合物进行脱蛋白处理。将无蛋白提取物在反相柱（Nucleosil 100 - 5 C18）上进行分离，并通过荧光法（激发波长275 nm，发射波长405 nm）进行定量。流动相由250 ml乙腈和750 ml含有10 mmol/l磷酸四丁铵的蒸馏水混合而成。尿液用0.25 mol/l磷酸按1:20（v/v）稀释，并用氢氧化钠溶液将其pH值调至3.6。稀释后的尿液样品在阳离子交换柱（Nucleosil 100 - 5 SA）上进行分离，同样通过荧光法进行检测。曲伐沙星能与内源性化合物充分分离。给出了验证结果。该方法已成功应用于健康志愿者的研究。
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