Therapeutics Research Centre, School of Medicine, The University of Queensland, Woolloongabba, QLD 4102, Australia.
J Pharm Biomed Anal. 2012 Mar 25;62:220-3. doi: 10.1016/j.jpba.2012.01.007. Epub 2012 Jan 15.
A simple and rapid high performance liquid chromatographic method was developed, validated and applied for the simultaneous determination of marbofloxacin (MBX) and trovafloxacin (TVX) in sheep plasma. Samples were extracted with 20% perchloric acid and MBX and TVX were separated on a C(18) column using a gradient mobile phase system consisting of 17.5mM of NaH(2)PO(4) and 1.5mM of tetrabutylammonium hydroxide aqueous solution, pH 3 (A) and 50% acetonitrile and 50% methanol (B), with UV detection at 293 and 270 nm. The retention times of MBX and TVX were 4.9 and 6.6 min respectively. The detection and quantification limits for MBX and TVX were 2 ng/mL and 10 ng/mL respectively for both compounds. The calibration curves were linear over a concentration range of 10-50,000 ng/mL for both antibiotics. The linearity, precision, accuracy, recovery and stability of the assay were evaluated from spiked sheep plasma. The method was successfully applied to sheep plasma samples obtained from MBX and TVX pharmacokinetic studies.
建立并验证了一种简单快速的高效液相色谱法，用于同时测定绵羊血浆中的马波沙星（MBX）和托氟沙星（TVX）。样品用 20%高氯酸提取，MBX 和 TVX 用 C(18)柱分离，流动相梯度为 17.5mM 的 NaH(2)PO(4)和 1.5mM 的四丁基氢氧化铵水溶液（pH3，A）和 50%乙腈-50%甲醇（B），在 293nm 和 270nm 处进行紫外检测。MBX 和 TVX 的保留时间分别为 4.9 和 6.6min。MBX 和 TVX 的检测限和定量限均为 10ng/ml，两者的线性范围均为 10-50000ng/ml。该方法从添加的绵羊血浆中评估了测定的线性、精密度、准确度、回收率和稳定性。该方法成功应用于马波沙星和托氟沙星药代动力学研究中获得的绵羊血浆样品。
