De Veylder L, de Almeida Engler J, Burssens S, Manevski A, Lescure B, Van Montagu M, Engler G, Inzé D
Department of Plant Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), Universiteit Gent, Belgium.
Planta. 1999 Jun;208(4):453-62. doi: 10.1007/s004250050582.
D-type cyclins are believed to regulate the onset of cell division upon mitogenic signaling. Here, the isolation is reported of a new D-type cyclin gene (CYCD4;1) of Arabidopsis thaliana (L.) Heynh. during a two-hybrid screen using the cyclin-dependent kinase CDC2aAt as bait. Transcription of CYCD4;1 can be induced by sucrose. The co-regulated expression of CYCD4;1 and CDC2aAt in starved suspension cultures upon mitogenic stimulation indicates that the formation of a complex between these two partners is important for the resumption of cell division activity. By in-situ hybridizations CYCD4;1 was shown to be expressed during vascular tissue development, embryogenesis, and formation of lateral root primordia. Expression during the latter process suggests that the induced expression of D-type cyclins by mitogenic stimuli might be one of the rate-limiting events for the initiation of lateral roots.
D型细胞周期蛋白被认为在有丝分裂信号作用下调节细胞分裂的起始。本文报道了在以细胞周期蛋白依赖性激酶CDC2aAt为诱饵进行的双杂交筛选过程中，从拟南芥中分离出一个新的D型细胞周期蛋白基因（CYCD4;1）。CYCD4;1的转录可被蔗糖诱导。在有丝分裂刺激下，饥饿悬浮培养物中CYCD4;1和CDC2aAt的共同调节表达表明，这两个蛋白之间形成复合物对于恢复细胞分裂活性很重要。通过原位杂交显示，CYCD4;1在维管组织发育、胚胎发生和侧根原基形成过程中表达。在后者过程中的表达表明，有丝分裂刺激诱导D型细胞周期蛋白的表达可能是侧根起始的限速事件之一。
