Wu Hua, Zheng Renhua, Hao Zhaodong, Meng Yan, Weng Yuhao, Zhou Xiaohong, Zhu Liming, Hu Xiangyang, Wang Guibin, Shi Jisen, Chen Jinhui
Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Fujian Academy of Forestry, Fuzhou 350012, China.
Plants (Basel). 2019 Nov 18;8(11):520. doi: 10.3390/plants8110520.
Phytosulfokine-α (PSK-α) is a newly discovered short peptide that acts as a phytohormone in various plants. Previous studies have shown that PSK-α is critical for many biological processes in plants, such as cell division and differentiation, somatic embryogenesis, pollen germination and plant resistance. In this study, we cloned two PSK homolog genes from (Lamb.) Hook (Chinese fir), and , and characterized their function in root development. Quantitative RT-PCR analyses showed that both and were expressed in vegetative organs, mainly in roots. Transgenic plants overexpressing or showed a higher frequency of adventitious root formation and increased root length. The expression of genes in that are involved in stem cell activity (, and ), radial organization of the root ( and ) and cell cycle (, , and ) were significantly up-regulated, which may contribute to the elongation of the primary root and the formation of adventitious root in transgenic lines. Our results suggest that play an important role during root growth and development.
植物磺肽素-α（PSK-α）是一种新发现的短肽，在多种植物中作为植物激素发挥作用。先前的研究表明，PSK-α对植物中的许多生物学过程至关重要，如细胞分裂和分化、体细胞胚胎发生、花粉萌发和植物抗性。在本研究中，我们从杉木（Cunninghamia lanceolata (Lamb.) Hook）中克隆了两个PSK同源基因CnPSK1和CnPSK2，并对它们在根系发育中的功能进行了表征。定量RT-PCR分析表明，CnPSK1和CnPSK2在营养器官中均有表达，主要在根中。过表达CnPSK1或CnPSK2的转基因拟南芥植株不定根形成频率更高，根长增加。参与干细胞活性（PLT1、PLT2和WOX5）、根的径向组织（SHR和SCR）和细胞周期（CYCB1;1、CYCD3;1、CYCA2;3和CYCA3;1）的拟南芥基因表达显著上调，这可能有助于转基因株系中主根的伸长和不定根的形成。我们的结果表明，CnPSK1和CnPSK2在根的生长和发育过程中发挥重要作用。
