Saenz A J, Petersen C E, Valentine N B, Gantt S L, Jarman K H, Kingsley M T, Wahl K L
Battelle Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Rapid Commun Mass Spectrom. 1999;13(15):1580-5. doi: 10.1002/(SICI)1097-0231(19990815)13:15<1580::AID-RCM679>3.0.CO;2-V.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used to demonstrate the reproducibility of bacterial spectra collected on different days. The reproducibility of analysis by MALDI-MS of intact Escherichia coli and Bacillus atrophaeus is presented as a replicate culture study in which spectra were collected on ten different occasions over a three-month period and by two different operators. The analysis resulted in the detection of specific biomarkers in the m/z 2000-20 000 range. Some of the peaks in the Escherichia coli spectra are identified by comparison with other published work. All of the spectra obtained are reproducible over the course of the experiment, but operator variability does exist. The Escherichia coli spectra show operator variability while the Bacillus atrophaeus spectra do not. This work demonstrates the utility of MALDI in obtaining consistent spectra from bacteria over a period of time.
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）用于证明在不同日期收集的细菌光谱的重现性。完整的大肠杆菌和萎缩芽孢杆菌的MALDI-MS分析重现性以重复培养研究的形式呈现，其中在三个月的时间内由两名不同的操作人员在十个不同的时间点收集光谱。分析结果是在m/z 2000 - 20000范围内检测到特定的生物标志物。通过与其他已发表的研究进行比较，确定了大肠杆菌光谱中的一些峰。在整个实验过程中获得的所有光谱都是可重现的，但操作人员的差异确实存在。大肠杆菌光谱显示出操作人员的差异，而萎缩芽孢杆菌光谱则没有。这项工作证明了MALDI在一段时间内从细菌中获得一致光谱的实用性。
