Holland R D, Wilkes J G, Rafii F, Sutherland J B, Persons C C, Voorhees K J, Lay J O
Food and Drugs Administration, National Center for Toxicological Research, Jefferson, AR 72079, USA.
Rapid Commun Mass Spectrom. 1996;10(10):1227-32. doi: 10.1002/(SICI)1097-0231(19960731)10:10<1227::AID-RCM659>3.0.CO;2-6.
Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identification of whole bacteria, either by comparison with archived reference spectra or by co-analysis with cultures of known bacteria. Bacteria were sampled from colonies on an agar plate, mixed with the matrix, air-dried, and introduced in batches into the mass spectrometer for analysis. In the first experiment, both bacterial strains that had been previously analyzed to obtain reference spectra and other strains that had not been analyzed were blind-numbered and their spectra were obtained. Those strains that matched reference spectra were found to be correctly identified. A second experiment involved co-analysis of reference strains and bind-numbered strains under identical conditions; species-specific identification was demonstrated by comparison of spectra of the blind-numbered strains with those of the standards. In all of the spectra obtained in these experiments, each bacterial strain showed a few characteristic high-mass ions which are thought to be derived from bacterial proteins. This work represents the first reported instance of successful bacterial chemotaxonomy by MALDI-TOFMS analysis of whole cells. For the strains tested, the method is rapid and simple.
基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOFMS）被作为一种快速鉴定完整细菌的方法进行了研究，该方法既可以与存档的参考光谱进行比较，也可以与已知细菌的培养物进行共分析。从琼脂平板上的菌落中采集细菌，与基质混合，空气干燥，然后分批引入质谱仪进行分析。在第一个实验中，对之前已分析以获得参考光谱的细菌菌株和未分析的其他菌株进行盲编号，并获取它们的光谱。发现那些与参考光谱匹配的菌株被正确鉴定。第二个实验涉及在相同条件下对参考菌株和盲编号菌株进行共分析；通过将盲编号菌株的光谱与标准菌株的光谱进行比较，证明了物种特异性鉴定。在这些实验中获得的所有光谱中，每个细菌菌株都显示出一些特征性的高质量离子，这些离子被认为源自细菌蛋白质。这项工作代表了首次通过对全细胞进行MALDI-TOFMS分析成功进行细菌化学分类的报道实例。对于所测试的菌株，该方法快速且简单。
