肠外营养脂质乳剂对中性粒细胞呼吸爆发的体外影响

In vitro influence of parenteral lipid emulsions on the respiratory burst of neutrophils.

作者信息

Heine J, Scheinichen D, Jaeger K, André M, Leuwer M

机构信息

Department of Anesthesiology, Hannover Medical School, Germany.

出版信息

Nutrition. 1999 Jul-Aug;15(7-8):540-5. doi: 10.1016/s0899-9007(99)00112-4.

DOI:10.1016/s0899-9007(99)00112-4

PMID:10422083

Abstract

The in vitro effect of a fish oil-derived lipid emulsion (omega-3) on the superoxide anion production during the respiratory burst (RB) of human neutrophils was compared to a LCT lipid (Intralipid), and an LCT/MCT emulsion (Lipofundin MCT). The effects of two concentrations (60 and 600 micrograms/mL) were evaluated by rhodamine in a flow cytometer. The RB was induced either by stimulation with Escherichia coli (E. coli) or by priming with TNF-alpha and FMLP stimulation. The results (mean +/- SD%, P < 0.05) were compared to positive control responses (RB without lipids). omega-3 (60 micrograms/mL, -8.2 [9.3]%; 600 micrograms/mL, -9.6 [11.1]%) and LCT (600 micrograms/mL, -8.0 [9.3]%) significantly suppressed the RB after stimulation with E. coli. LCT/MCT increased the RB after E. coli (60 micrograms/mL, 15.7 [15.4]%; 600 micrograms/mL, 42.7 [21.4]%) as well as after TNF-alpha/FMLP stimulation (600 micrograms/mL, 27.4 [23.7]%). The in vitro influence of parenteral lipid emulsions on the superoxide anion production of human neutrophils is dependent on the length of the fatty acid molecule.

摘要

将一种鱼油衍生的脂质乳剂（ω-3）在人中性粒细胞呼吸爆发（RB）期间对超氧阴离子产生的体外作用，与长链甘油三酯脂质乳剂（英脱利匹特）和长链甘油三酯/中链甘油三酯乳剂（力保肪宁MCT）进行了比较。通过在流式细胞仪中使用罗丹明评估了两种浓度（60和600微克/毫升）的作用。RB通过用大肠杆菌（E. coli）刺激或用肿瘤坏死因子-α（TNF-α）预处理和甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）刺激来诱导。将结果（平均值±标准差%，P<0.05）与阳性对照反应（无脂质的RB）进行比较。ω-3（60微克/毫升，-8.2 [9.3]%；600微克/毫升，-9.6 [11.1]%）和长链甘油三酯（600微克/毫升，-8.0 [9.3]%）在用大肠杆菌刺激后显著抑制了RB。长链甘油三酯/中链甘油三酯在用大肠杆菌刺激后（60微克/毫升，15.7 [15.4]%；600微克/毫升，42.7 [21.4]%）以及在TNF-α/FMLP刺激后（600微克/毫升，27.4 [23.7]%）增加了RB。肠外脂质乳剂对人中性粒细胞超氧阴离子产生的体外影响取决于脂肪酸分子的长度。