Wanten G J, Roos D, Naber A H
Department of Gastroenterology, University Hospital, Nijmegen, The Netherlands.
Clin Nutr. 2000 Oct;19(5):327-31. doi: 10.1054/clnu.2000.0113.
To test the hypothesis that structurally different lipid emulsions have distinct immunomodulatory properties, we analysed neutrophil migration in the presence of various lipid emulsions.
Neutrophils of 8 volunteers were pre-incubated in medium or physiological 2.5 mM emulsions containing long-chain (LCT), medium-chain (MCT), mixed LCT/MCT, alpha -tocopherol-enriched LCT/MCT (LCT/MCT-E) or structured triglycerides (SL). Thereafter, the cells were put on top of 3 microm-pore-sized cell culture filters and incubated for one hour in the presence or absence of a chemo-attractant. Neutrophil migration was measured as the percentage of cells that had passed the filter in the presence (chemotaxis) or absence (random migration) of a chemotactic factor.
Compared to lipid-free incubation (19+/-1%) random neutrophil migration significantly decreased with LCT/MCT (11+/-2%), LCT/MCT-E (12+/-2) and MCT (5+/-2%), while LCT (18+/-3%) and SL (20+/-1%) had no effect. N-formyl-methionyl-leucyl-phenylalanine- (fMLP, 10(-8)M) or zymosan-activated-serum-induced (ZAS, 10%) filter passage under lipid-free conditions amounted to 61+/-14% and 70+/-13%, respectively. These values decreased with LCT/MCT to 11+/-9% and 15+/-7%; with LCT/MCT-E to 18+/-10% and 28+/-12%; with SL to 39+/-18% and 57+/-14%, and with MCT to 5+/-2% and 10+/-6%, (all P<0.01), while LCT had no effect. Compared to LCT/MCT, the alpha -tocopherol-enriched formulation significantly increased ZAS- and fMLP-induced chemotaxis. fMLP-induced chemotaxis decreased in direct proportion to LCT/MCT triglyceride concentration.
Human neutrophil migration is distinctively inhibited by structurally different lipid emulsions, depending on triglyceride chain-length and concentration as well as alpha -tocopherol content.
为验证结构不同的脂质乳剂具有不同免疫调节特性这一假说，我们分析了在各种脂质乳剂存在的情况下中性粒细胞的迁移情况。
将8名志愿者的中性粒细胞在含有长链（LCT）、中链（MCT）、LCT/MCT混合、富含α-生育酚的LCT/MCT（LCT/MCT-E）或结构化甘油三酯（SL）的培养基或生理浓度2.5 mM乳剂中预孵育。此后，将细胞置于3微米孔径的细胞培养滤膜上，在有或无趋化因子的情况下孵育1小时。中性粒细胞迁移以在趋化因子存在（趋化作用）或不存在（随机迁移）的情况下通过滤膜的细胞百分比来衡量。
与无脂质孵育（19±1%）相比，LCT/MCT（11±2%）、LCT/MCT-E（12±2）和MCT（5±2%）使中性粒细胞随机迁移显著减少，而LCT（18±3%）和SL（20±1%）无此作用。在无脂质条件下，N-甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP，10⁻⁸M）或酵母聚糖激活血清（ZAS，10%）诱导的滤膜通过率分别为61±14%和70±13%。这些值在LCT/MCT作用下分别降至11±9%和15±7%；在LCT/MCT-E作用下分别降至18±10%和28±12%；在SL作用下分别降至39±18%和57±14%；在MCT作用下分别降至5±2%和10±6%（所有P<0.01），而LCT无作用。与LCT/MCT相比，富含α-生育酚的制剂显著增加ZAS和fMLP诱导的趋化作用。fMLP诱导的趋化作用与LCT/MCT甘油三酯浓度成反比降低。
根据甘油三酯链长度、浓度以及α-生育酚含量的不同，结构不同的脂质乳剂对人中性粒细胞迁移有明显抑制作用。
