Harris R D, Luff B J, Wilkinson J S, Piehler J, Brecht A, Gauglitz G, Abuknesha R A
Optoelectronics Research Centre, University of Southampton, UK.
Biosens Bioelectron. 1999 Apr 30;14(4):377-86. doi: 10.1016/s0956-5663(99)00014-7.
This paper presents the detailed design and characterisation of a regenerable integrated optical surface plasmon resonance immunoprobe as a detector for the triazine herbicide simazine. A sensor design theoretically optimised for use in the aqueous environment is presented and its fabrication described. Experimental results on the sensitivity to changes in bulk refractive index of the analyte and on non-specific binding of ovalbumin are presented. Binding inhibition immunoassays were conducted for simazine and the lower limit of detection determined to be 0.16 microgram/l using anti-simazine IgG antibodies and 0.11 microgram/l using anti-simazine Fab fragments. A sample test cycle of 20 min was established.
本文介绍了一种可再生集成光学表面等离子体共振免疫探针的详细设计与特性，该探针用作三嗪类除草剂西玛津的检测器。提出了一种理论上针对水性环境使用进行优化的传感器设计，并描述了其制造过程。给出了关于分析物本体折射率变化的灵敏度以及卵清蛋白非特异性结合的实验结果。对西玛津进行了结合抑制免疫测定，使用抗西玛津IgG抗体时检测下限确定为0.16微克/升，使用抗西玛津Fab片段时检测下限为0.11微克/升。建立了20分钟的样品测试周期。
