Khalaf-Allah S S
Department of Hygiene and Preventive Medicine, Faculty of Veterinary Medicine, Moshtohor, Benha University, Qalubia, Egypt.
Dtsch Tierarztl Wochenschr. 1999 Jun;106(6):248-51.
Forty Egyptian native cattle calves of 4-6 months old randomly allocated into two groups of twenty animals each were used to assess the effect of immunization of animals with a recombinant Bm86 antigen derived from Boophilus microplus ticks on induction of immunity that could protect calves during tick season. The immunization protocol involved two injections administered intramuscularly, the first was applied with complete Freund's adjuvant and the second was given with incomplete Freund's adjuvant two months later. Control calves were given saline plus adjuvant. Immunization reduced the number of adult ticks developing from a subsequent challenge infestation by 78% in immunized calves. Vaccination also, significantly reduced the weight of adult ticks in immunized calves (30.51%). The results of skin delayed hypersensitivity reaction revealed that the diameter of sites injected with the recombinant Bm86 antigen was significantly larger in immunized calves than those in controls. Analysis of the immune response indicated that there was a significant increase in the level of IgG and IgA antibodies in serum of immunized calves and protection from reinfestation was correlated with the levels of circulating antibodies.
40头4至6月龄的埃及本地牛犊被随机分为两组，每组20头，用于评估用来源于微小牛蜱的重组Bm86抗原免疫动物对诱导免疫力的效果，这种免疫力可在蜱虫季节保护牛犊。免疫方案包括两次肌肉注射，第一次注射使用完全弗氏佐剂，两个月后第二次注射使用不完全弗氏佐剂。对照牛犊注射生理盐水加佐剂。免疫使免疫后的牛犊因后续攻毒感染而发育的成年蜱数量减少了78%。接种疫苗还显著降低了免疫牛犊体内成年蜱的重量（30.51%）。皮肤迟发型超敏反应结果显示，注射重组Bm86抗原部位的直径在免疫牛犊中显著大于对照牛犊。免疫反应分析表明，免疫牛犊血清中IgG和IgA抗体水平显著升高，免受再次感染的保护作用与循环抗体水平相关。
