Watanabe F, Oki Y, Ozawa M, Masuzawa M, Iwabuchi M, Yoshimi T, Nishiguchi T, Iino K, Sasano H
Department of Medicine, Hamamatsu University School of Medicine, Japan.
Peptides. 1999;20(2):205-9. doi: 10.1016/s0196-9781(98)00175-2.
Plasma immunoreactive (IR-) urocortin (Ucn) and corticotropin-releasing factor (CRF) levels in pregnant women were measured by their specific radioimmunoassays after extraction. Although plasma IR-CRF levels were increased in pregnant women as compared to men and non-pregnant women, there was no difference of plasma IR-Ucn levels among groups. Ucn mRNA was detected in cytotrophoblasts and syncytiotrophoblasts by in situ hybridization. A reverse-phase high-performance liquid chromatography (HPLC) showed the major peak of IR-Ucn in placenta and plasma that had similar chromatographic mobility to synthetic Ucn1-40. These data suggest that Ucn is produced and processed into the same form of synthetic Ucn in placenta, but not secreted into maternal blood.
采用特异性放射免疫分析法，在提取后测定孕妇血浆中免疫反应性（IR-）尿皮质素（Ucn）和促肾上腺皮质激素释放因子（CRF）的水平。与男性和未孕女性相比，孕妇血浆IR-CRF水平升高，但各组间血浆IR-Ucn水平无差异。通过原位杂交在细胞滋养层细胞和合体滋养层细胞中检测到Ucn mRNA。反相高效液相色谱（HPLC）显示，胎盘和血浆中IR-Ucn的主峰与合成Ucn1-40具有相似的色谱迁移率。这些数据表明，Ucn在胎盘中产生并加工成与合成Ucn相同的形式，但不会分泌到母体血液中。
