Takahashi K, Totsune K, Sone M, Murakami O, Satoh F, Arihara Z, Sasano H, Iino K, Mouri T
Department of Molecular Biology, Tohoku University School of Medicine, Sendai, Miyagi, Japan.
Peptides. 1998;19(4):643-7. doi: 10.1016/s0196-9781(98)00012-6.
Regional distribution of urocortin-like immunoreactivity (UCN-LI) in the human brain was studied by radioimmunoassay and was compared with that of corticotropin-releasing hormone (CRH). In addition, the expression of UCN mRNA was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) method. UCN-LI was detected in every region of brain examined, including hypothalamus, pons, cerebral cortex, and cerebellum. The concentrations of UCN-LI in the human brain were approximately 3 pmol/g wet weight in any brain region, and no marked regional difference was noted. On the other hand, the highest concentrations of CRH-LI were found in the frontal cortex, temporal cortex, and hypothalamus and the lowest in the pons. Reverse phase high-performance liquid chromatography of the UCN-LI in the human brain extract showed two immunoreactive peaks; one peak eluting earlier and one in the position of synthetic human UCN. RT-PCR showed that UCN mRNA was expressed in every region of brain examined. These findings indicated that UCN and UCN mRNA were widely expressed in the human brain.
通过放射免疫分析法研究了人脑中尿皮质素样免疫反应性（UCN-LI）的区域分布，并将其与促肾上腺皮质激素释放激素（CRH）的区域分布进行了比较。此外，采用逆转录聚合酶链反应（RT-PCR）法检测了UCN mRNA的表达。在所检测的脑的各个区域，包括下丘脑、脑桥、大脑皮层和小脑中均检测到了UCN-LI。人脑中UCN-LI的浓度在任何脑区约为3 pmol/g湿重，未发现明显的区域差异。另一方面，CRH-LI的最高浓度出现在额叶皮层、颞叶皮层和下丘脑，最低浓度出现在脑桥。人脑提取物中UCN-LI的反相高效液相色谱显示出两个免疫反应峰；一个峰洗脱较早，另一个峰在合成人UCN的位置。RT-PCR显示UCN mRNA在所检测的脑的各个区域均有表达。这些发现表明UCN和UCN mRNA在人脑中广泛表达。
