Borromeo J R, Koshy N, Park W M, Xia S, Hardy K, Tilson M D
Department of Surgery, St. Luke's-Roosevelt Hospital Center, New York, New York, 10019, USA.
J Surg Res. 1999 Aug;85(2):217-24. doi: 10.1006/jsre.1999.5659.
We have reported the sequences of four novel proteins derived from extracts of human aortic tissue and a cDNA library from human aortic adventitia. These proteins are immunoreactive with serum immunoglobulins from patients with abdominal aortic aneurysms (AAA), and they have homologies of amino acid sequence with microfibrillar proteins of connective tissue. We are reporting separately that two of these proteins are artery-specific antigenic proteins (ASAPs) in man. The present work investigates the regional distribution of these two proteins (AAAP-40 and MatCAM-1) in mouse (E-beta-b). Antibodies were raised in rabbit against polypeptides encoding novel amino acid sequences, unique to these proteins (e.g., not reported in GenBank). Immunohistochemical studies with these two specific antibodies show conspicuous immunoreactivity of collagen-associated microfibrils in the aortic adventitia of the murine abdominal and thoracic aorta. Immunoreactive peptides were not present in brain, muscle, or kidney. These findings support the hypothesis that proteins occur in the mouse that are homologous to a unique family of aortic microfibrillar proteins in man.
我们已经报道了从人主动脉组织提取物和人主动脉外膜cDNA文库中获得的四种新型蛋白质的序列。这些蛋白质与腹主动脉瘤（AAA）患者的血清免疫球蛋白发生免疫反应，并且它们在氨基酸序列上与结缔组织的微纤维蛋白具有同源性。我们另行报道了其中两种蛋白质是人动脉特异性抗原蛋白（ASAPs）。目前的工作研究了这两种蛋白质（AAAP - 40和MatCAM - 1）在小鼠（E - beta - b）中的区域分布。用针对这些蛋白质特有的新氨基酸序列（例如，GenBank中未报道）的多肽在兔中制备抗体。用这两种特异性抗体进行的免疫组织化学研究显示，在小鼠腹主动脉和胸主动脉的外膜中，胶原相关微纤维有明显的免疫反应性。脑、肌肉或肾脏中不存在免疫反应性肽。这些发现支持这样的假设，即小鼠中存在与人主动脉微纤维蛋白独特家族同源的蛋白质。
